PL EN


Preferences help
enabled [disable] Abstract
Number of results
2007 | 54 | 4 | 883-886
Article title

Limitation of usage of PicoGreen dye in quantitative assays of double-stranded DNA in the presence of intercalating compounds

Content
Title variants
Languages of publication
EN
Abstracts
EN
PicoGreen is a very sensitive fluorescent dye for quantitative assays of double-stranded DNA (dsDNA) in solution and is used in several analytical protocols in which sensitive and precise DNA detection is needed, also for examination of drug-DNA interactions. The data shown in this paper indicate that compounds intercalating to DNA influence the applicability of PicoGreen dye for quantitative measurements of dsDNA, and for this reason PicoGreen dye is not suitable for examination of drug-DNA interactions, especially interstrand DNA crosslinks.
Year
Volume
54
Issue
4
Pages
883-886
Physical description
Dates
published
2007
received
2007-09-09
revised
2007-11-20
accepted
2007-12-09
(unknown)
2007-12-10
References
  • Ashley N, Harris D, Poulton J (2005) Exp Cell Res 303: 432-446.
  • Choi SJ, Szoka FC (2000) Anal Biochem 281: 95-97.
  • Cosa G, Focsaneanu K-S, McLean JRN, McNamee JP, Scaiano JC (2001) Photochem Photobiol 73: 585-599.
  • Dorr RT, Bowden GT, Alberts DS, Liddil JD (1985) Cancer Res 45: 3510-3516.
  • Gagna CE, Kuo HR, Chan NJ, Mitacek EJ, Spivak A, Pasquariello TD, Balgobin C, Mukhi R, Lambert WC (2007) J Histochem Cytochem 55: 999-1014.
  • Guillo C, Ferrance JP, Landers JP (2006) J Chromatogr A 1113: 239-243.
  • Hein I, Flekna G, Wagner M, Nocker A, Camper AK (2006) Appl Environ Microbiol 72: 6860-6862.
  • Jenkins TC (1997) Drug-DNA Interaction Protocols. In Methods in Molecular Biology, vol 90, Fox KR, eds, p 195. Humana Press Inc., Totowa.
  • Kasaisavee V, Suwanarusk R, Nosten F, Kyle DE, Barrends M, Jones J, Price R, Russell B, Lek-Uthai U (2006) Exp Parasitol 114: 34-39.
  • Koba M, Konopa J (2007) Acta Biochim Polon 54: 297-306.
  • Kral T, Widerak K, Langner M, Hof M (2005) J Fluoresc 15: 179-183.
  • Markovits J, Roques BP, LePecq J-B (1979) Anal Biochem 94: 259-264.
  • Murakami P, McCaman MT (1999) Anal Biochem 274: 283-288.
  • Romppanen EL, Salvolainen K, Mononen I (2000) Anal Biochem 279: 111-114.
  • Singer VL, Jones LJ, Yue ST, Haugland RP (1997) Anal Biochem 249: 228-238.
  • Skladanowski A, Konopa J (1994) Biochem Pharmacol 47: 2269-2278.
  • Skladanowski A, Konopa J (2000) Brit J Cancer 82: 1300-1304.
  • Skladanowski A, Koba M, Konopa J (2001) Biochem Pharmacol 61: 67-72.
  • Tolun G, Myers RS (2003) Nucleic Acids Res 31: e111.
  • Zipper H, Brunner H, Bernhagen J, Vitzthum F (2004) Nucleic Acids Res 32: e103.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv54p883kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.