Analysis of gypsogenin saponins in homeopathic tinctures
Languages of publication
A relatively simple and short procedure for the quantitative determination of gypsogenin saponins was performed to evaluate homeopathic tinctures in which those compounds can be regarded as one of the active constituents. This method comprises partial hydrolysis of saponins, subsequent extraction of liberated prosaponin (gypsogenin 3-O-glucuronide) and its analysis by high performance liquid chromatography. Glycyrrhizic acid was used as an internal standard. This method was successfully applied to the analysis of mother tinctures obtained from Saponaria officinalis. Thus, the determination of triterpenoid saponins can be used as a convenient and sufficient method of standardization of selected homeopathic tinctures.
- Ansel HC, Popovich NG, Allen LV (1995) Pharmaceutical dosage forms and drug delivery systems; pp 120-125. Williams & Wilkins, Malvern.
- Bilia AR, Bergonzi MC, Gallori S, Mazzi G, Vinceri FF (2002a) J Pharm Biomed Anal 30: 613-624.
- Bilia AR, Bergonzi MC, Mazzi G, Vinceri FF (2002b) Drug Dev Ind Pharm 28: 609-619.
- Bouguet-Bonnet S, Rochd M, Mutzenhardt P, Henry M (2002) Magn Res Chem 40: 618-621.
- Directive 2001/83/EC and 2004/27/EC of the European Parliament and of the Council on the Community code relating to medicinal products for human use.
- EMEA/HMPWG/25/99, EMEA/CPMP/ICH/281/95, http://www.eudra.org.emea.html
- Eudra/Q/92/021: 'Note for Guidance on stability testing of new active substances and medicinal products'.
- Frye J C (2003) Seminars in Integrative Medicine 1: 158-166.
- Henry M, Pauthe-Dayde D, Rochd M (1989) J Chromatogr 477: 413-415.
- Lyakina MN (2004) Pharm Chem J 38: 28-30.
- Nandi M (1999) Br Homeopath J 88: 161-165.
- Oleszek WA (2002) J Chromatogr A 967: 147-162.
- Selivantchikova IB, Lyakina MN, Kostennikova ZP (2001) Farmatsija 6: 14-15.
- Satti J (2005) Seminars in Integrative Medicine 3: 113-122.
- Sparg SG, Light ME, van Staden J (2004) J Ethnopharmacol 94: 219-243.
Publication order reference