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2007 | 54 | 4 | 783-796
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Structural and functional characteristics of two novel members of pathogensis-related multigene family of class 10 from yellow lupine+

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PR-10 proteins (pathogensis-related), ubiquitous within the plant kingdom, are usually encoded by multigene families. To date we have identified 10 homologous pr-10 genes in a yellow lupine cDNA library. Here, the structure and expression of two newly identified yellow lupine pr-10 genes (LlYpr10-2b and LlYpr10-2f) are presented. Many potential regulatory sites were found in both gene promoters including common ones as well as those unique for each gene. However, promoter deletion analysis in transgenic tobacco plants revealed similar patterns of reporter gene (gus) expression. Shortened fragments of both gene promoters studied caused high GUS activity in leaves (along vascular bundles), stamen stigma, anthers and pollen grains. When conjugated with longer LlYpr-10.2 promoter fragments, GUS was additionally present in petal edges. Only a long fragment of the LlYpr10-2b gene promoter caused GUS expression in the stem. In yellow lupine the pr-10.2 genes are present in all studied organs, but their level of expression depends on the stage of development and is affected by wounding, oxidative stress and salicylic acid treatment. Silencing of the Llpr-10.2b gene in 4-week-old yellow lupine plants did not lead to any visible symptoms, which suggests that the function of the silenced gene is supplemented by its close homologues, still present in the studied plants.
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  • Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  • Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  • Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  • Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  • Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  • Akiyama Y, Onizuka K, Noguchi T, Ando M (1998) Parallel Protein Information Analysis (PAPIA) System Running on a 64-Node PC Cluster. Genome Informatics 9: 131-140 (http: //
  • Bais HP, Vepachedu R, Lawrence CB, Stermitz FR, Vivanco JM (2003) Molecular and biochemical characterization of an enzyme responsible for the formation of hypericin in St. John's Wort (Hypericum perforatum L.). J Biol Chem 278: 32413-32422.
  • Balsamo RA, Wang JL, Eckard KJ, Wang C-S, Lord EM (1995) Immunogold localization of a developmentally regulated, tapetal-specific, 15 kDa lily anther protein. Protoplasma 189: 17-25.
  • Bantignies B, Seguin J, Muzac I, Dedaldechamp F, Gulick P, Ibrahim R (2000) Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots. Plant Mol Biol 42: 871-881.
  • Baulcombe D (2005) RNA silencing. Trends Biochem Sci 30: 290-293.
  • Beuning L, Bowen J, Persson H, Barraclough D, Bulley S, MacRae EA (2004) Characterisation of Mal d 1-related genes in Malus. Plant Mol Biol 55: 369-388.
  • Breda C, Sallaud Ch, El-Turk J, Buffard D, de Kozak I, Esnault R, Kondorosi A (1996) Defense reaction in Medicago sativa: a gene encoding a class 10 PR protein is expressed in vascular bundles. Mol Plant Microbe Interact 9: 713-719.
  • Bufe A, Spangfort MD, Kahlert H, Schlaak M, Becker W-M (1996) The major birch pollen allergen, Bet v 1, shows ribonuclease activity. Planta 199: 413-415.
  • Chadha P, Das RH (2006) A pathogensis related protein, AhPR10 from peanut: an insight of its mode of antifungal activity. Planta 225: 213-222.
  • Chen W, Provart NJ, Glazebrook J, Katagiri F, Chang HS, Eulgem T, Mauch F, Luan S, Zou G, Whitham SA et al. (2002) Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. Plant Cell 14: 559-574.
  • Colditz F, Niehaus K, Krajinski F (2007) Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta 226: 57-71.
  • Crowell DN, John ME, Russel D, Amasino M (1992) Characterization of a stress-induced, developmentally regulated gene family from soybean. Plant Mol Biol 18: 459-466
  • Eulgem T, Rushton PJ, Robatzek S, Somssich IE (2000) The WRKY superfamily of plant transcription factors. Trends Plant Sci 5: 199-205.
  • Eulgem T, Rushton PJ, Schmelzer E, Hahlbrock K, Somssich IE (1999) Early nuclear events in plant defense signaling: rapid gene activation by WRKY transcription factors. EMBO J 18: 4689-4699.
  • Finkler C, Giacomet C, Muschner VC, Salzano FM, Freitas LB (2005) Molecular investigations of pathogensis-related Bet v1 homologues in Passiflora (Passifloraceae). Genetica 124: 117-125.
  • Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811.
  • Gamas P, de Billy F, Truchet G (1998) Symbiosis-specific expression of two Medicago truncatula nodulin genes, MtN1 and MtN13, encoding products homologous to plant defense proteins. MPMI 11: 393-403.
  • Handschuh L, Femiak I, Kasperska A, Sikorski MM (2003) Pathogensis-Related Proteins of Subclass PR-10.2 in Yellow Lupine - Comprehensive Analysis, Proceedings of the XIIth International Congress on Genes, Gene Families and Isozymes, Berlin, pp157-160.
  • Heinemeyer T, Wingender E, Reuter I, Hermjakob H, Kel AE, Kel OV, Ignatieva EV, Ananko EA, Podkolodnaya OA, Kolpakov FA, Podkolodny NL, Kolchanov NA (1998) Databases on Transcriptional Regulation: TRANSFAC, TRRD, and COMPEL. Nucleic Acids Res. 26: 362-367.
  • Hoffmann-Sommergruber K, Vanek-Krebitz M, Radauer Ch, Wen J, Ferreira F, Scheiner O, Breitender H (1997) Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogensis-related proteins share intron positions. Gene 197: 91-100.
  • Huang J-C, Chang F-C, Wang C-S (1997) Characterization of a lily tapetal transcript that shares sequence similarity with a class of intracellular pathogensis-related (IPR) proteins. Plant Mol Biol 34: 681-686.
  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907.
  • Jwa N-S, Agrawal GK, Rakwal R, Park C-H, Agrawal VP (2001) Molecular cloning and characterization of a novel jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves. Biochem Biophys Res Commun 286: 973-983.
  • Kunkel BN, Brooks DM (2002) Cross talk between signaling pathways in pathogen defense. Curr Opin Plant Biol 5: 325-331.
  • Liscombe DK, MacLeod BP, Loukanina N, Nandi OI, Facchini PJ (2005) Evidence for the monophyletic evolution of benzylisoquinoline alkaloid biosynthesis in angiosperms. Phytochemistry 66: 1374-1393.
  • Liu J-J, Ekramoddoullah AK (2003) Root-specific expression of a western white pine PR10 gene is mediated by different promoter regions in transgenic tobacco. Plant Mol Biol 52: 103-120.
  • Liu JJ, Ekramoddoullah AK (2004) Characterization, expression and evolution of two novel subfamilies of Pinus monticola cDNAs encoding pathogensis-related (PR)-10 proteins. Tree Physiol 24: 1377-1385.
  • McGee JD, Hamer JE, Hodges TK (2001) Characterization of a PR-10 pathogensis-related gene family induced in rice during infection with Magnaporthe grisea. Mol Plant Microbe Interact 14: 877-886.
  • Meister G, Tuschl T (2004) Mechanisms of gene silencing by double-stranded RNA. Nature 431: 343-349.
  • Mishra NS, Tuteja R, Tuteja N (2006) Signaling through MAP kinase networks in plants. Arch Biochem Biophys 452: 55-68.
  • Moiseyev GP, Beintema JJ, Fedoreyeva LI, Yakovlev GI (1994) High sequence similarity between a ribonuclease from ginseng calluses and fungus-elicited proteins from parsley indicates that intracellular pathogensis-related proteins are ribonucleases. Planta 193: 470-472.
  • Moiseyev GP, Fedoreyeva LI, Zhuravlev YN, Yasnetskaya E, Jekel PA, Beintema JJ (1997) Primary structures of two ribonucleases from ginseng calluses. New members of the PR-10 family of intracellular pathogensis-related plant proteins. FEBS Lett 407: 207-210.
  • Park C-J, Kim K-J, Shin R, Park JM, Shin J-C, Paek K-H (2004) Pathogensis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway. Plant J 37: 186-198.
  • Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M (2006) Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin Plant Cell 18: 2622-2634.
  • Pinto MP, Ricardo CPP (1995) Lupinus albus L. pathogensis-related proteins that show similarity to PR-10 proteins. Plant Physiol 109: 1345-1351.
  • Pozueta-Romero J, Klein M, Houlne G, Schantz M-L, Meyer B, Schantz R (1995) Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): identification of a new family of transposable elements. Plant Mol Biol 28: 1011-1025.
  • Pühringer H, Moll D, Hoffmann-Sommergruber K, Watillon B, Katinger H, Laimer da Camara Machado M (2000) The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogen-inducible. Plant Sci 152: 35-50.
  • Rakwal R, Agrawal GK, Yonekura M (2001) Light-dependent induction of OsPR10 in rice (Oryza sativa L.) seedlings by the global stress signaling molecule jasmonic acid and protein phosphatase 2A inhibitors. Plant Sci 161: 469-479.
  • Schenk MF, Gilissen LJ, Esselink GD, Smulders MJ (2006) Seven different genes encode a diverse mixture of isoforms of Betv 1, the major birch pollen allergen. BMC Genomics 7: 168.
  • Schug J (2003) Using TESS to predict transcription factor binding sites in dna sequence. In Current Protocols in Bioinformatics, Baxevanis, Andreas D, eds, Wiley J and Sons (publisher) (
  • Shah J (2003) The salicylic acid loop in plant defense. Curr Opin Plant Biol 6: 365-371.
  • Sikorski MM (1997) Expression of Lupinus luteus cDNA coding for PR10 protein in Escherichia coli: Purification of the recombinant protein for structural and functional studies. Acta Biochim Polon 44: 565-578.
  • Sikorski MM, Szlagowska AE, Legocki AB (1998) Structure of Lupinus luteus genes Ypr10.1a and Ypr10.1b encoding two homologues of pathogenesis-related proteins of PR10 class. Plant Physiol 116: 1192-1195.
  • Sikorski MM, Biesiadka J, Kasperska AE, Kopcińska J, Łotocka B, Golinowski W, Legocki AB (1999) Expression of genes encoding PR10 class pathogensis-related proteins is inhibited in yellow lupine root nodules. Plant Sci 149: 125-137.
  • Sikorski MM, Stępkowski T, Świderska A, Biesiadka J, Łotocka B, Kopcińska J, Golinowski W, Legocki AB (1999) Differential expression of symbiosis-related genes in yellow lupine. In Highlights of Nitrogen Fixation Research, pp 125-130. Plenum Publishing Corporation, New York.
  • Sikorski MM, Handschuh L, Biesiadka J, Legocki AB (2000) Two subclasses of yellow lupine PR10 proteins and their possible function during the symbiosis development. Curr Plant Sci Biotech Agriculture 38: 319-322.
  • Silverstein KA, Graham MA, Van den Bosch KA (2006) Novel paralogous gene families with potential function in legume nodule sand seeds. Curr Opin Plant Biol 9: 142-146.
  • Singh KB, Foley RC, Onate-Sanchez L (2002) Transcription factors in plant defense and stress response. Curr Opin Plant Biol 5: 430-436.
  • Somssich IE, Schmelzer E, Bollmann J, Hahlbrock K (1986) Rapid activation by fungal elicitor of genes encoding 'pathogensis-related' proteins in cultured parsley cells. Proc Natl Acad Sci USA 83: 2427-2430.
  • Somssich IE, Schmelzer E, Kawalleck P, Hahlbrock K (1988) Gene structure and in situ transcript localization of pathogensis-related protein 1 in parsley. Mol Gen Genet 213: 93-98.
  • Strömvik MW, Sundararaman VP, Vodkin LO (1999) A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs. Plant Mol Biol 41: 217-231.
  • Swoboda I, Hoffman-Sommergruber K, O'Riordain G, Scheiner O, Heberle-Bors E, Vicente O (1996) Bet v 1 proteins, the major birch pollen allergens and members of a family of conserved pathogensis-related proteins, show ribonuclease activity in vitro. Physiol Plantar 96: 433-438.
  • Szweykowska-Kulińska Z, Jarmolowski A, Figlerowicz M (2003) RNA interference and its role in the regulation of eucaryotic gene expression. Acta Biochim Polon 50: 217-229.
  • Ślesak I, Libik M, Karpinska B, Karpinski S, Miszalski Z (2007) The role of hydrogen peroxide in regulation of plant metabolism and cellular signaling in response to environmental stresses. Acta Biochim Polon 54: 39-50.
  • Tijsterman M, Ketting RF, Plasterk RHA (2002) The genetics of RNA silencing. Annu Rev Genet 36: 489-519.
  • Trinh H, Barker D, Ratet P (2001) Regeneration and transformation methods in the Manuals of The EMBO Practical course on the New Plant Model System Medicago truncatula, Module 2, organized by Pascal Ratet.
  • Turck F, Zhou A, Somssich IE (2004) Stimulus-dependent, promoter-specific binding of transcription factor WRKY1 to its native promoter and the defense-related gene PcPR1-1 in parsley. Plant Cell 16: 2573-2585.
  • van Loon LC, van Strien EA (1999) The families of pathogensis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol Mol Plant Pathol 55: 85-97.
  • Vanek-Krebitz M, Hoffmann-Sommergruber K, Laimer da Câmara Machado M, Susani M, Ebner C, Kraft D, Scheiner O, Breitender H (1995) Cloing and sequencing of Mal d 1, the major allergen from apple (Malus domestica) and its immunological relationship to Bet v 1, the major birch pollen allergen. Biochem Biophys Res Commun 214: 538-551.
  • Walter MH, Liu J-W, Wünn J, Hess D (1996) Bean ribonuclease-like pathogensis-related protein genes (Ypr10) display complex patterns of developmental, dark-induced and exogenous-stimulus-dependent expression. Eur J Biochem 239: 281-293.
  • Wang C-S, Huang J-C, Hu J-H (1999) Characterization of two subclasses of PR-10 transcripts in lily anthers and induction of their genes through separate signal transduction pathways. Plant Mol Biol 40: 807-814.
  • Wu F, Yan M, Li Y, Chang S, Song X, Zhou Z, Gong W (2003) cDNA cloning, expression, and mutagenesis of a PR-10 protein SPE-16 from the seeds of Pachyrrhizus erosus. Biochem Biophys Res Commun 312: 761-766.
  • Yanagisawa S (2002) The Dof family of plant transcription factors. Trends Plant Sci 7: 555-560.
  • Yanagisawa S, Schmidt RJ (1999) Diversity and similarity among recognition sequences of Dof transcription factors. Plant J 17: 209-214.
  • Zhou X-J, Lu S, Xu Y-H, Wang J-W, Chen X-Y (2002) A cotton cDNA (GaPR-10) encoding a pathogensis-related 10 protein with in vitro ribonuclease activity. Plant Sci 162: 629-636.
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