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2007 | 54 | 4 | 747-755
Article title

Polyadenylation and decay of 26S rRNA as part of Nicotiana tabacum response to cadmium

Content
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EN
Abstracts
EN
In contrast to mRNAs, ribosomal RNAs are generally not considered to be polyadenylated. Only a few recent reports describe non-abundant polyadenylated rRNA-related transcripts that have been detected and characterized in yeast and in human cells. Here we depict the phenomenon of 26S rRNA polyadenylation and degradation that was observed in shoots of Nicotiana tabaccum plants grown in the presence of cadmium. Fragments corresponding to 26S rRNA were identified using suppression subtractive hybridization during screening for genes induced in tobacco plants upon a three-week exposure to 15 µM cadmium chloride. Extracts prepared from the above-ground tissues of cadmium-treated tobacco plants were supposed to contain exclusively polyadenylated mRNAs. Surprisingly, numerous polyadenylated fragments matching parts of 26S rRNA were identified and their presence was confirmed by Northern blot and cDNA amplification techniques. To our knowledge this is the first report on rRNA polyadenylation in plants.
Year
Volume
54
Issue
4
Pages
747-755
Physical description
Dates
published
2007
received
2007-07-25
revised
2007-09-17
accepted
2007-09-28
(unknown)
2007-12-08
References
  • Basile A, Alba di Nuzzo R, Capasso C, Sorbo S, Capasso A, Carginale V (2005) Effect of cadmium on gene expression in the liverwort Lunularia cruciata. Gene 356: 153-159.
  • Benavides MP, Gallego SM, Tomaro ML (2005) Cadmium toxicity in plants. Braz J Plant Physiol 17: 21-34.
  • Clemens S, Palmgren MG, Kramer U (2002) A long way ahead: understanding and engineering plant metal accumulation. Trends Plant Sci 7: 309-315.
  • Clemens S, Schroeder JI, Degenkolb T (2001) Caenorhabditis elegans expresses a functional phytochelatin synthase. Eur J Biochem 268: 3640-3643.
  • Crawford DR, Lauzon RJ, Wang Y, Mazurkiewicz JE, Schools GP, Davies KJ (1997) 16S mitochondrial ribosomal RNA degradation is associated with apoptosis. Free Radic Biol Med 22: 1295-1300.
  • Deckert J (2005) Cadmium toxicity in plants: is there any analogy to its carcinogenic effect in mammalian cells? Biometals 18: 475-481.
  • Dreyfus M, Regnier P (2002) The poly(A) tail of mRNAs: bodyguard in eukaryotes, scavenger in bacteria. Cell 111: 611-613.
  • Fleischmann J, Liu H (2001) Polyadenylation of ribosomal RNA by Candida albicans. Gene 265: 71-76.
  • Fojtova M, Kovarik A (2000) Genotoxic effect of cadmium is associated with apoptotic changes in tobacco cells. Plant Cell Environ 23: 531-537.
  • Fusco N, Micheletto L, Dal Corso G, Borgato L, Furini A (2005) Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator Brassica juncea L. J Exp Bot 56: 3017-3027.
  • Herrin DL, Schmidt GW (1988) Rapid, reversible staining of northern blots prior to hybridization. Biotechniques 6: 196-200.
  • Hoat TX, Nakayashiki H, Tosa Y, Mayama S (2006) Specific cleavage of ribosomal RNA and mRNA during victorin-induced apoptotic cell death in oat. Plant J 46: 922-933.
  • Houge G, Doskeland SO, Boe R, Lanotte M (1993) Selective cleavage of 28S rRNA variable regions V3 and V13 in myeloid leukemia cell apoptosis. FEBS Lett 315: 16-20.
  • Houge G, Robaye B, Eikhom TS, Golstein J, Mellgren G, Gjertsen BT, Lanotte M, Doskeland SO (1995) Fine mapping of 28S rRNA sites specifically cleaved in cells undergoing apoptosis. Mol Cell Biol 15: 2051-2062.
  • King KL, Jewell CM, Bortner CD, Cidlowski JA (2000) 28S ribosome degradation in lymphoid cell apoptosis: evidence for caspase and Bcl-2-dependent and -independent pathways. Cell Death Differ 7: 994-1001.
  • Kuai L, Fang F, Butler JS, Sherman F (2004) Polyadenylation of rRNA in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 101: 8581-8586.
  • Lafarga M, Lerga A, Andres MA, Polanco JI, Calle E, Berciano MT (1997) Apoptosis induced by methylazoxymethanol in developing rat cerebellum: organization of the cell nucleus and its relationship to DNA and rRNA degradation. Cell Tissue Res 289: 25-38.
  • Lam E, Pontier D, del Pozo O (1999) Die and let live — programmed cell death in plants. Curr Opin Plant Biol 2: 502-507.
  • Legg PD, Collins GB, Litton CC (1970) Registration of LA Burley 21 tobacco germplasm. Crop Sci 10: 212.
  • Li W, Dickman MB (2004) Abiotic stress induces apoptotic-like features in tobacco that is inhibited by expression of human Bcl-2. Biotechnol Lett 26: 87-95.
  • Louie M, Kondor N, DeWitt JG (2003) Gene expression in cadmium-tolerant Datura innoxia: detection and characterization of cDNAs induced in response to Cd^2+. Plant Mol Biol 52: 81-89.
  • Martelli A, Rousselet E, Dycke C, Bouron A, Moulis JM (2006) Cadmium toxicity in animal cells by interference with essential metals. Biochimie 88: 1807-1814.
  • Mendoza-Cozatl DG, Moreno-Sanchez R (2006) Control of glutathione and phytochelatin synthesis under cadmium stress. Pathway modeling for plants. J Theor Biol 238: 919-936.
  • Mendoza-Cozatl D, Loza-Tavera H, Hernandez-Navarro A, Moreno-Sanchez R (2005) Sulfur assimilation and glutathione metabolism under cadmium stress in yeast, protists and plants. FEMS Microbiol Rev 29: 653-671.
  • Minglin L, Yuxiu Z, Tuanyao C (2005) Identification of genes up-regulated in response to Cd exposure in Brassica juncea L. Gene 363: 151-158.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473-493.
  • Ortega-Villasante C, Rellan-Alvarez R, Del Campo FF, Carpena-Ruiz RO, Hernandez LE (2005) Cellular damage induced by cadmium and mercury in Medicago sativa. J Exp Bot 56: 2239-2251.
  • Pulido MD, Parrish AR (2003) Metal-induced apoptosis: mechanisms. Mutat Res 533: 227-241.
  • Rubinelli P, Siripornadulsil S, Gao-Rubinelli F, Sayre RT (2002) Cadmium- and iron-stress-inducible gene expression in the green alga Chlamydomonas reinhardtii: evidence for H43 protein function in iron assimilation. Planta 215: 1-13.
  • Samali A, Gilje B, Doskeland SO, Cotter TG, Houge G (1997) The ability to cleave 28S ribosomal RNA during apoptosis is a cell-type dependent trait unrelated to DNA fragmentation. Cell Death Differ 4: 289-293.
  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
  • Sanita di Toppi L, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41: 105-130.
  • Sharma SS, Dietz KJ (2006) The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 57: 711-726.
  • Slomovic S, Laufer D, Geiger D, Schuster G (2006) Polyadenylation of ribosomal RNA in human cells. Nucleic Acids Res 34: 2966-2975.
  • Sugiura M, Sakaki T, Yabusaki Y, Ohkawa H (1996) Cloning and expression in Escherichia coli and Saccharomyces cerevisiae of a novel tobacco cytochrome P-450-like cDNA. Biochim Biophys Acta 1308: 231-240.
  • Suzuki N, Koizumi N, Sano H (2001) Screening of cadmium-responsive genes in Arabidopsis thaliana. Plant Cell Environ 24: 1177-1188.
  • Tong YP, Kneer R, Zhu YG (2004) Vacuolar compartmentalization: a second-generation approach to engineering plants for phytoremediation. Trends Plant Sci 9: 7-9.
  • van Doorn WG, Woltering EJ (2005) Many ways to exit? Cell death categories in plants. Trends Plant Sci 10: 117-122.
  • Wawrzynska A, Lewandowska M, Hawkesford MJ, Sirko A (2005) Using a suppression subtractive library-based approach to identify tobacco genes regulated in response to short-term sulphur deficit. J Exp Bot 56: 1575-1590.
  • Wawrzynski A, Kopera E, Wawrzynska A, Kaminska J, Bal W, Sirko A (2006) Effects of simultaneous expression of heterologous genes involved in phytochelatin biosynthesis on thiol content and cadmium accumulation in tobacco plants. J Exp Bot 57: 2173-2182.
  • Yakimova ET, Kapchina-Toteva VM, Laarhoven LJ, Harren FM, Woltering EJ (2006) Involvement of ethylene and lipid signalling in cadmium-induced programmed cell death in tomato suspension cells. Plant Physiol Biochem 44: 581-589.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv54p747kz
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