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2007 | 54 | 4 | 703-715
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Hepatitis C - new developments in the studies of the viral life cycle

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Hepatitis C virus (HCV) is a causative agent of chronic liver disease leading to cirrhosis, liver failure and hepatocellular carcinoma. The prevalence of HCV is estimated as 3% of the world population and the virus is a major public health problem all over the world. For over 16 years, since HCV had been discovered, studies of the mechanisms of the viral life cycle and virus-host interactions have been hampered by the lack of a cell culture system allowing the virus to be grown in laboratory conditions. However, in recent years some new model systems to study HCV have been developed. The major breakthrough of the last two years was the cell culture system for maintaining the virus in an adapted hepatocyte-derived cell line. This review describes the techniques and applications of most of the in vitro systems and animal models currently used for working with hepatitis C virus.
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  • Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
  • Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
  • Agnello V, Abel G, Elfahal M, Knight GB, Zhang QX (1999) Hepatitis C virus and other Flaviviridae viruses enter cells via low density lipoprotein receptor. Proc Natl Acad Sci USA 96: 12766-12771.
  • Andre P, Komurian-Pradel F, Deforges S, Perret M, Berland JL, Sodoyer M, Pol S, Brechot C, Paranhos-Baccala G, Lotteau V (2002) Characterization of low- and very-low-density hepatitis C virus RNA-containing particles. J Virol 76: 6919-6928.
  • Andre P, Perlemuter G, Budkowska A, Brechot C, Lotteau V (2005) Hepatitis C virus particles and lipoprotein metabolism. Semin Liver Dis 25: 93-104.
  • Bartenschlager R, Lohmann V (2000) Replication of hepatitis C virus. J Gen Virol 81: 1631-1648.
  • Bartenschlager R, Kaul A, Sparacio S (2003) Replication of the hepatitis C virus in cell culture. Antiviral Res 60: 91-102.
  • Bartenschlager R, Frese M, Pietschmann T (2004) Novel insights into hepatitis C virus replication and persistence. Adv Virus Res 63: 71-180.
  • Bartosch B, Bukh J, Meunier JC, Granier C, Engle RE, Blackwelder WC, Emerson SU, Cosset FL, Purcell RH (2003a) In vitro assay for neutralizing antibody to hepatitis C Virus: evidence for broadly conserved neutralization epitopes. Proc Natl Acad Sci USA 100: 14199-14204.
  • Bartosch B, Dubuisson J, Cosset FL (2003b) Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes. J Exp Med 197: 633-642.
  • Bartosch B, Vitelli A, Granier C, Goujon C, Dubuisson J, Pascale S, Scarselli E, Cortese R, Nicosia A, Cosset FL (2003c) Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-BI scavenger receptor. J Biol Chem 278: 41624-41630.
  • Bassett SE, Thomas DL, Brasky KM, Landford RE (1999) Viral persistence, antibody to E1 and E2, and hypervariable region 1 sequence stability in hepatitis C virus-inoculated chimpanzees. J Virol 73: 1118-1122.
  • Bertolini L, Iacovacci S, Ponzetto A, Gorini G, Battaglia M, Carloni G (1993) The human bone-marrow-derived B-cell line CE, susceptible to hepatitis C virus infection. Res Virol 144: 281-285.
  • Blight KJ, Kolyhakov AA, Rice CM (2000) Efficient initiation of HCV RNA replication in cell culture. Science 290: 1972-1974.
  • Blight KJ, McKeating JA, Marcotrigiano J, Rice CM (2003) Efficient replication of hepatitis C virus genotype 1a RNAs in cell culture. J Virol 77: 3181-3190.
  • Blight KJ, McKeating JA, Rice CM (2002) Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication. J Virol 76: 13001-13014.
  • Boni S, Lavergne JP, Boulant S, Cahour A (2005) Hepatitis C virus core protein acts as a trans-modulating factor on internal translation initiation of the viral RNA. J Biol Chem 280: 17737-17748.
  • Boulant S, Becchi M, Penin F, Lavergne JP (2003) Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b. J Biol Chem 278: 45785-45792.
  • Bradley DW (2000) Studies of non-A, non-B hepatitis and characterization of the hepatitis C virus in chimpanzees. Curr Top Microbiol Immunol 242: 1-23.
  • Brass V, Moradpour D, Blum HE (2006) Molecular virology of hepatitis C virus (HCV): 2006 update Int J Med Sci 3: 29-34.
  • Bright H, Carroll AR, Watts PA, Fenton RJ (2004) Development of a GB virus B marmoset model and its validation with a novel series of hepatitis C virus NS3 protease inhibitors. J Virol 78: 2062-2071.
  • Bretner M (2005) Existing and future therapeutic options for hepatitis C virus infection. Acta Biochim Polon 52: 57-70.
  • Bukh J, Apgar CL, Yanagi M (1999) Toward a surrogate model for hepatitis C virus: an infectious molecular clone of the GB virus-B hepatitis agent. Virology 262: 470-478.
  • Bukh J, Forns X, Emerson SU, Purcell RH (2001) Studies of hepatitis C virus in chimpanzees and their importance for vaccine development. Intervirology 44: 132-142.
  • Choo QL, Kuo G, Weiner AJ, Overby LR, Bradley DW, Houghton M (1989) Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244: 359-362.
  • Cocquerel L, Voisset C, Dubuisson J (2006) Hepatitis C virus entry: potential receptors and their biological functions. J Gen Virol 87: 1075-1084.
  • Cribier B, Schmitt C, Bingen A, Kirn A, Keller F (1995) In vitro infection of peripheral blood mononuclear cells by hepatitis C virus. J Gen Virol 76: 2485-2491
  • Custer RP, Bosma GC, Bosma MJ (1985) Severe combined immunodeficiency (SCID) in the mouse. Am J Pathol 120: 464-477.
  • Date T, Kato T, Miyamoto M, Zhao Z, Yasui K, Mizokami M, Wakita T (2004) Genotype 2a hepatitis C virus subgenomic replicon can replicate in HepG2 and IMY-N9 cells. J Biol Chem 279: 22371-22376.
  • Dash S, Halim AB, Tsuji H, Hiramatsu N, Gerber MA (1997) Transfection of HepG2 cells with infectious hepatitis C virus genome. Am J Pathol 151: 363-373.
  • Deleersnyder V, Pillez A, Wychowski C, Blight K, Xu J, Hahn YS, Rice CM, Dubuisson J (1997) Formation of native hepatitis C virus glycoprotein complexes. J Virol 71: 697-704.
  • Diaz O, Delers F, Maynard M, Demignot S, Zoulim F, Chambaz J, Trepo C, Lotteau V, Andre P (2006) Preferential association of hepatitis C virus with apolipoprotein B48-containing lipoproteins. J Gen Virol 87: 2983-2991.
  • Diedrich G (2006) How does hepatitis C virus enter cells? FEBS J 273: 3871-3885.
  • Dreux M, Pietschmann T, Granier C, Voisset C, Ricard-Blum S, Mangeot PE, Keck Z, Foung S, Vu-Dac N, Dubuisson J, Bartenschlager R, Lavillette D, Cosset FL (2006) High density lipoprotein inhibits hepatitis C virus neutralising antibodies by stimulating cell entry via activation of the scavenger receptor B1. J Biol Chem 281: 18285-18295.
  • Drummer HE, Maerz A, Poumbourios P (2003) Cell surface expression of functional hepatitis C virus E1 and E2 glycoproteins. FEBS Lett 546: 385-390.
  • Dubuisson J (2000) Folding, assembly and subcellular localisation of hepatitis C virus glycoproteins. Curr Top Microbiol Immunol 242: 135-148.
  • Dubuisson J, Hsu HH, Cheung RC, Greenberg HB, Russel DG, Rice CM (1994) Formation and intracellular localisation of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia and Sindbis viruses. J Virol 68: 6147-6160.
  • Fournier C, Sureau C, Coste J, Ducos J, Pageaux G, Larrey D, Domergue J, Maurel P (1998) In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus. J Gen Virol 79: 2367-2374.
  • Gale M, Beard MR (2001) Molecular colnes of hepatitics C virus: applications to animal models. ILAR J 42: 139-151.
  • Gastaminza P, Kapadia SB, Chisari FV (2006) Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles. J Virol 80: 11074-11081.
  • Goffard A, Callens N, Bartosch B, Wychowski C, Cosset FL, Montpellier C, Dubuisson J (2005) Role of N-linked glycans in the functions of hepatitis C virus envelope glycoproteins. J Virol 79: 8400-8409.
  • Gosert R, Egger D, Lohmann V, Bartenschlager R, Blum HE, Bienz K, Moradpour D (2003) Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons. J Virol 77: 5487-5492.
  • Griffin SD, Beales LP, Clarke DS, Worsfold O, Evans SD, Jaeger J, Harris MP, Rowlands DJ (2003) The p7 protein of hepatitis C virus forms an ion channel that is blocked by the antiviral drug, Amantadine. FEBS Lett 535: 34-38.
  • Hardy RW, Marcotrigiano J, Blight KJ, Majors JE, Rice CM (2003) Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells. J Virol 77: 2029-2037.
  • He Y, Yan W, Coito C, Li Y, Gale M Jr, Katze MG (2003) The regulation of hepatitis C virus (HCV) internal ribosome entry site-mediated translation by HCV replicons and nonstructural proteins. J Gen Virol 84: 535-543.
  • Hope RG, Murphy DJ, McLauchlan J (2002) The domains required to direct core proteins of hepatitis C virus and GB virus-B to lipid droplets share common features with plant oleosin proteins. J Biol Chem 277: 4261-4270.
  • Hsu EC, Hsi B, Hirota-Tsuchihara M, Ruland J, Iorio C, Sarangi F, Diao J, Migliaccio G, Tyrrell DL, Kneteman N, Richardson CD (2003a) Modified apoptotic molecule (BID) reduces hepatitis C virus infection in mice with chimeric human livers. Nat Biotechnol 21: 519-525.
  • Hsu M, Zhang J, Flint M, Logvinoff C, Cheng-Mayer C, Rice CM, McKeating JA (2003b) Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Proc Natl Acad Sci USA 100: 7271-7276.
  • Iacovacci S, Sargiacomo M, Parolini I, Ponzetto A, Peschle C, Carloni G (1993) Replication and multiplication of hepatitis C virus genome in human foetal liver cells. Res Virol 144: 275-279.
  • Ikeda M, Sugiyama K, Mizutani T, Tanaka T, Tanaka K, Sekihara H, Shimotohno K, Kato N (1998) Human hepatocyte clonal cell lines that support persistent replication of hepatitis C virus. Virus Res 56: 157-167.
  • Ikeda M, Yi M, Li K, Lemon SM (2002) Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hepatitis C virus replicate efficiently in cultured Huh7 cells. J Virol 76: 2997-3006.
  • Ilan E, Arazi J, Nussbaum O, Zauberman A, Eren R, Lubin I, Neville L, Ben-Moshe O, Kischitzky A, Litchi A, Margalit I, Gopher J, Mounir S, Cai W, Daudi N, Eid A, Jurim O, Czerniak A, Galun E, Dagan S (2002) The hepatitis C virus (HCV)-Trimera mouse: a model for evaluation of agents against HCV. J Infect Dis 185: 153-161.
  • Kato N, Ikeda M, Mizutani T, Sugiyama K, Noguchi M, Hirohashi S, Shimotohno K (1996) Replication of hepatitis C virus in cultured non-neoplastic human hepatocytes. Jpn J Cancer Res 87: 787-792.
  • Kato T, Furusaka A, Miyamoto M, Date T, Yasui K, Hiramoto J, Nagayama K, Tanaka T, Wakita T (2001) Sequence analysis of hepatitis C virus isolated from a fulminant hepatitis patient. J Med Virol 64: 334-339.
  • Kato T, Date T, Miyamoto M, Furusaka A, Tokushige K, Mizokami M, Wakita T (2003) Efficient replication of the genotype 2a hepatitis C virus subgenomic replicon. Gastroenterology 125: 1808-1817.
  • Kato T, Date T, Miyamoto M, Zhao Z, Mizokami M, Wakita T (2005) Nonhepatic cell lines HeLa and 293 support efficient replication of the hepatitis C virus genotype 2a subgenomic replicon. J Virol 79: 592-596.
  • Kneteman NM, Weiner AJ, O'Connell J, Collett M, Gao T, Aukerman L, Kovelsky R, Ni Z-J, Hashash A, Kline J, Hsi B, Schiller D, Douglas D, Tyrrell DL, Mercer FD (2006) Anti-HCV therapies in chimeric scid-Alb/uPA mice parallel outcomes in human clinical application. Hepatology 43: 1346-1353.
  • Kolykhalov AA, Agapov EV, Blight KJ, Mihalik K, Feinstone SM, Rice CM (1997) Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA. Science 277: 570-574.
  • Krieger N, Lohmann V, Bartenschlager R (2001) Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations. J Virol 75: 4614-4624.
  • Lai VC, Dempsey S, Lau JY, Hong Z, Zhong W (2003) In vitro RNA replication directed by replicase complexes isolated from the subgenomic replicon cells of hepatitis C virus. J Virol 77: 2295-2300.
  • Lanford RE, Sureau C, Jacob JR, White R, Fuerst TR (1994) Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR. Virology 202: 606-614.
  • Lanford RE, Bigger C, Bassett S, Klimpel G (2001) The chimpanzee model of hepatitis C virus infections. ILAR J 42: 117-126.
  • Laskus T, Radkowski M, Wang L-F, Cianciara J, Vargas H, Rakela J (1997) Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: a case against extrahepatic replication. J Gen Virol 78: 2747-2750.
  • Lin C, Gates CA, Rao BG, Brennan DL, Fulghum JR, Luong YP, Frantz JD, Lin K, Ma S, Wei YY, Perni RB, Kwong AD (2005) In vitro studies of cross-resistance mutations against two hepatitis C virus serine protease inhibitors, VX-950 and BILN 2061. J Biol Chem 280: 36784-36791.
  • Lindenbach BD, Evans MJ, Syder AJ, Wolk B, Tellinghuisen TL, Liu CC, Maruyama T, Hynes RO, Burton DR, McKeating JA, Rice CM (2005) Complete replication of hepatitis C virus in cell culture. Science 309: 623-626.
  • Lindenbach BD, Meuleman P, Ploss A, Vanwolleghem T, Syder AJ, McKeating JA, Lanford RE, Feinstone SM, Major ME, Leroux-Roels G, Rice CM (2006) Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro. Proc Natl Acad Sci USA 103: 3805-3809.
  • Lohmann V, Korner F, Koch JO, Herian U, Theilmann L, Bartenschlager R (1999) Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science 285: 110-113.
  • Lohmann V, Korner F, Dobierzewska A, Bartenschlager R (2001) Mutations in hepatitis C virus RNAs conferring cell culture adaptation. J Virol 75: 1437-1449.
  • Lozach PY, Lortat-Jacob H, de Lacroix de Lavalette A, Staropoli I, Foung S, Amara A, Houles C, Fieschi F, Schwartz O, Virelizier JL, Arenzana-Seisdedos F, Altmeyer R (2003) DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2. J Biol Chem 278: 20358-20366.
  • Lozach PY, Amara A, Bartosch B, Virelizier JL, Arenzana-Seisdedos F, Cosset FL, Altmeyer R (2004) C-type lectins L-SIGN and DC-SIGN capture and transmit infectious hepatitis C virus pseudotype particles. J Biol Chem 279: 32035-32045.
  • MacParland SA, Pham TNQ, Gujar SA, Michalak TI (2006) De novo infection and propagation of wild-type hepatitis C virus in human T lymphocytes in vitro. J Gen Virol 87: 3577-3586.
  • Ma H, Leveque V, De Witte A, Li W, Hendricks T, Clausen SM, Cammack N, Klump K (2005) Inhibition of native hepatitis C virus replicase by nucleotide and nonnucleoside inhibitors. Virology 332: 8-15.
  • Maillard P, Krawczynski K, Nitkiewicz J, Bronnert C, Sidorkiewicz M, Gounon P, Dubuisson J, Faure G, Crainic R, Budkowska A (2001) Nonenveloped nucleocapsids of hepatitis C virus in the serum of infected patients. J Virol 75: 8240-8250.
  • Maillard P, Huby T, Andréo U, Moreau M, Chapman J, Budkowska A (2006) The interaction of natural hepatitis C virus with human scavenger receptor SR-BI/Cla1 is mediated by ApoB-containing lipoproteins. FASEB J 20: 735-737.
  • Major ME, Feinstone SM (2000) Characterization of hepatitis C virus infectious clones in chimpanzees. Curr Top Microbiol Immunol 242: 279-298.
  • Masciopinto F, Giovani C, Campagnoli S, Galli-Stampino L, Colombatto P, Brunetto M, Yen TSB, Houghton M, Pileri P, Abrignani S (2004) Association of hepatitis C virus envelope proteins with exosomes. Eur J Immunol 34: 2834-2842.
  • McHutchison JG, Bartenschlager R, Patel K, Pawlotsky JM (2006) The face of future hepatitis C antiviral drug development: recent biological and virologic advances and their translation to drug development and clinical practice. J Hepatol 44: 411-421.
  • Mercer DF, Schiller DE, Elliott JF, Douglas DN, Hao C, Rinfret A, Addison WR, Fisher KP, Churchill TA, Lakey JR, Tyrrell DL, Kneteman NM (2001) Hepatitis C virus replication in mice with chimeric human livers. Nat Med 7: 927-933.
  • Meunier JC, Engle RE, Faulk K, Zhao M, Bartosch B, Alter H, Emerson SU, Cosset FL, Purcell RH, Bukh J (2005) Evidence for cross-genotype neutralization of hepatitis C virus pseudo-particles and enhancement of infectivity of apolipoprotein C1. Proc Natl Acad Sci USA 102: 4560-4565.
  • Mizutani T, Kato N, Hirota M, Sugiyama K, Murakami A, Shimotohno K (1995) Inhibition of hepatitis C virus replication by antisense oligonucleotide in culture cells. Biochem Biophys Res Commun 212: 906-911.
  • Mizutani T, Kato N, Ikeda M, Sugiyama K, Shimotohno K (1996a) Long-term human T-cell culture system supporting hepatitis C virus replication. Biochem Biophys Res Commun 227: 822-826.
  • Mizutani T, Kato N, Saito S, Ikeda M, Sugiyama K, Shimotohno K (1996b) Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2. J Virol 70: 7219-7223.
  • Monazahian M, Bohme I, Bonk S, Koch A, Scholz C, Grethe S, Thomssen R (1999) Low density lipoprotein receptor as a candidate receptor for hepatitis C virus. J Med Virol 57: 223-229.
  • Moradpour D, Evans MJ, Gosert R, Yuan Z, Blum HE, Goff SP, Lindenbach BD, Rice CM (2004) Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes. J Virol 78: 7400-7409.
  • Nakajima N, Hijikata M, Yoshikura H, Shimizu YK (1996) Characterization of long-term cultures of hepatitis C virus. J Virol 70: 3325-3329.
  • Op De Beeck A, Dubuisson J (2003) Another putative receptor for hepatitis C virus. Hepatology 37: 705-707.
  • Op De Beeck A, Voisset C, Bartosch B, Ciczora Y, Cocquerel L, Keck Z, Foung S, Cosset FL, Dubuisson J (2004) Characterization of functional hepatitis C virus envelope glycoproteins. J Virol 78: 2994-3002.
  • Owsianka AM, Timms JM, Tarr AW, Brown RJ, Hickling TP, Szwejk A, Bienkowska-Szewczyk K, Thomson BJ, Patel AH, Ball JK (2006) Identification of conserved residues in the E2 envelope glycoprotein of the hepatitis C virus that are critical for CD81 binding. J Virol 80: 8695-8704.
  • Patel J, Patel AH, McLauchlan J (2001) The transmembrane domain of the hepatitis C virus E2 glycoprotein is required for correct folding of the E1 glycoprotein and native complex formation. Virology 279: 58-68.
  • Pavlovic D, Neville DC, Argaud O, Blumberg B, Dwek RA, Fischer WB, Zitzmann N (2003) The hepatitis C virus p7 protein forms an ion channel that is inhibited by long-alkyl-chain iminosugar derivatives. Proc Natl Acad Sci USA 100: 6104-6108.
  • Pawlowska M, Palewicz E, Halota W (2006) Early virologic response in therapy with pegylated interferon alpha-2B plus ribavirin in children with chronic hepatitis C. Przegl Epidemiol 60: 71-77 (in polish).
  • Pham TN, Macparland SA, Coffin CS, Lee SS, Bursey FR, Michalak TI (2005) Mitogen-induced upregulation of hepatitis C virus expression in human lymphoid cells. J Gen Virol 86: 657-666.
  • Pestova TV, Shatsky IN, Fletcher SP, Jackson RT, Hellen CUT (1998) A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initiation of hepatitis C and classical swine fever virus RNAs. Genes Dev 12: 67-83 .
  • Pietschmann T, Lohmann V, Rutter G, Kurpanek K, Bartenschlager R (2001) Characterization of cell lines carrying self-replicating hepatitis C virus RNAs. J Virol 75: 1252-1264.
  • Pietschmann T, Lohmann V, Kaul A, Krieger N, Rinck G, Rutter G, Strand D, Bartenschlager R (2002) Persistent and transient replication of full-length hepatitis C virus genomes in cell culture. J Virol 76: 4008-4021.
  • Pietschmann T, Kaul A, Koutsoudakis G, Shavinskaya A, Kallis S, Steinmann E, Abid K, Negro F, Dreux M, Cosset FL, Bartenschlager R (2006) Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras. Proc Natl Acad Sci USA 103: 7408-7413.
  • Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner AJ, Houghton M, Rosa D, Grandi G, Abrignani S (1998) Binding of hepatitis C virus to CD81. Science 282: 938-941.
  • Puerstinger G, Paeshuyse J, De Clercq E, Neyts J (2007) Antiviral 2,5-distributed imidazo[4,5-c]pyridines: from anti-pestivirus to anti hepatitis C activity. Bioorg Med Chem Lett 17: 390-393.
  • Revie D, Alberti MO, Braich RS, Chelyapov N, Bayles D, Prichard JG, Salahuddin SZ (2006) Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies. Virol J 3: 81.
  • Rumin S, Berthillon P, Tanaka E, Kiyosawa K, Trabaud M-A, Bizollon T, Gouillat C, Gripon P, Guguen-Guillouzo C, Inchauspe G, Trepo C (1999) Dynamic analysis of hepatitis C virus replication and quasispecies selection in long-term cultures of adult human hepatocytes infected in vitro. J Gen Virol 80: 3007-3018.
  • Sbardellati A, Scarselli E, Verschoor E, De Tomassi A, Lazzaro D, Traboni C (2001) Generation of infectious and transmissible virions from a GB virus B full-length consensus clone in tamarins J Gen Virol 82: 2437-2448.
  • Scarselli E, Ansuini H, Cerino R, Roccasecca RM, Acali S, Filocamo G, Traboni C, Nicosia A, Cortese R, Vitelli A (2002) The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J 21: 5017-5025.
  • Seipp S, Muller HM, Pfaff E, Stremmel W, Theilmann L, Goeser T (1997) Establishment of persistent hepatitis C virus infection and replication in vitro. J Gen Virol 78: 2467-2476.
  • Shimizu Y, Iwamoto A, Hijikata M, Purcell RH, Yoshikura H (1992) Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proc Natl Acad Sci USA 89: 5477-5481.
  • Shimizu YK, Purcell RH, Yoshikura H (1993) Correlation between the infectivity of hepatitis C virus in vivo and its infectivity in vitro. Proc Natl Acad Sci USA 90: 6037-6041.
  • Shimizu YK, Yoshikura H (1994) Multicycle infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons. J Virol 68: 8406-8408.
  • Shimizu YK, Hijikata M, Iwamoto A, Alter HJ, Purcell RH, Yoshikura H (1994) Neutralizing antibodies against hepatitis C virus and the emergence of neutralization escape mutant viruses. J Virol 68: 1494-1500.
  • Shimizu YK, Igarashi H, Kiyohara T, Cabezon T, Farci P, Purcell RH, Yoshikura H (1996) In vitro assay for neutralizing antibody to hepatitis C virus: Evidence for broadly conserved neutralization epitopes. Virology 223: 409-412.
  • Shimizu YK, Igarashi H, Kiyohara T, Shapiro M, Wong DC, Purcell RH, Yoshikura H (1998) Infection of a chimpanzee with hepatitis C virus grown in cell culture. J Gen Virol 79: 1383-1386.
  • Simmonds P (2004) Genetic diversity and evolution of hepatitis C virus — 15 years on. J Gen Virol 85: 3173-3188.
  • Song ZQ, Hao F, Min F, Ma QY, Liu GD (2001) Hepatitis C virus infection of human hepatoma cell line 7721 in vitro. World J Gastroenterol 7: 685-689.
  • Spahn CM, Kieft JS, Grassucci RA, Penczek PA, Zhou K, Doudna JA, Frank J (2001) Hepatitis C virus IRES RNA-induced changes in the conformation of the 40s ribosomal subunit. Science 291: 1959-1962.
  • Sugiyama K, Kato N, Mizutani T, Iked M, Tanaka T, Shimotohno K (1997) Genetic analysis of the hepatitis C virus (HCV) genome from HCV-infected human T cells. J Gen Virol 78: 329-336.
  • Sung VM, Shimodaira S, Doughty AL, Picchio GR, Can H, Yen TS, Lindsay KL, Levine AM, Lai MM (2003) Establishment of B-cell lymphoma cell lines persistently infected with hepatitis C virus in vivo and in vitro: the apoptotic effects of virus infection. J Virol 77: 2134-2146.
  • Thomson M, Nascimbeni M, Havert MB, Major M, Gonzales S, Alter H, Feinstone SM, Murthy KK, Rehermann B, Liang TJ (2003) The clearance of hepatitis C virus infection in chimpanzees may not necessarily correlate with the appearance of acquired immunity. J Virol 77: 862-870.
  • Thomssen R, Bonk S, Thiele A (1993) Density heterogeneities of hepatitis c virus in human sera due to binding of beta-lipoproteins and immunoglobulins. Med Microbiol Immunol 182: 329-334.
  • Tscherne DM, Jones CT, Evans MJ, Lindenbach BD, McKeating JA, Rice CM (2006) Time- and temperature-dependent activation of hepatitis C virus for low-pH-triggered entry. J Virol 80: 1734-1741.
  • Tsukiyama-Kohara K, Iizuka N, Kohara M, Nomoto A (1992) Internal ribosome entry site within hepatitis C virus RNA. J Virol 66: 1476-1483.
  • Valli MB, Bertolini L, Iacovacci S, Ponzetto A, Carloni G (1995) Detection of a 5´ UTR variation in the HCV genome after a long-term in vitro infection. Res Virol 146: 285-288.
  • Varaklioti A, Vassilaki N, Georgopoulou U, Mavromara P (2002) Alternate translation occurs within the core coding region of the hepatitis C viral genome. J Biol Chem 277: 17713-17721.
  • Voisset C, Callens N, Blanchard E, Op De Beeck A, Dubuisson J, Vu-Dac N (2005) High density lipoproteins facilitate hepatitis C virus entry through the scavenger receptor class B type I. J Biol Chem 280: 7793-7799.
  • Vrolijk JM, Kaul A, Hansen BE, Lohman V, Haagmans BL, Schlam SW, Bartenschlager R (2003) A replicon-based bioassay for the measurment of interferons in patients with chronic hepatitis C. J Virol Methods 110: 201-210.
  • Wakita T, Pietschmann T, Kato T, Date T, Miyamoto M, Zhao Z, Murthy K, Habermann A, Krausslich HG, Mizokami M, Bartenschlager R, Liang TJ (2005) Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 11: 791-796.
  • Walewski JL, Keller TR, Stump DD, Branch AD (2001) Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame. RNA 7: 710-721.
  • WHO report (2003) Hepatitis C guide. (published on-line,
  • Xu Z, Choi J, Yen TS, Lu W, Strohecker A, Govindarajan S, Chien D, Selby MJ, Ou J (2001) Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. EMBO J 20: 3840-3848.
  • Yanagi M, Purcell RH, Emerson SU, Bukh J (1997) Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee. Proc Natl Acad Sci USA 94: 8738-8743.
  • Zhong J, Gastaminza P, Cheng G, Kapadia S, Kato T, Burton DR, Wieland SF, Uprichard SL, Wakita T, Chisari FV (2005) Robust hepatitis C virus infection in vitro. Proc Natl Acad Sci USA 102: 9294-9299.
  • Zhu Q, Oei Y, Mendel DB, Garrett EN, Patawaran MB, Hollenbach PW, Aukerman SL, Weiner AJ (2006) Novel robust hepatitis C virus mouse efficacy model. Antimicrob Agents Chemother 50: 3260-3268.
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