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2007 | 54 | 3 | 671-672
Article title

A freeze-thaw method for disintegration of Escherichia coli cells producing T7 lysozyme used in pBAD expression systems

Content
Title variants
Languages of publication
EN
Abstracts
EN
The pLysN plasmid containing the T7 lysozyme gene under control of the lac promoter was constructed to facilitate cell disintegration after expression of recombinant proteins in arabinose-induced expression systems. The usefulness of this plasmid was tested in Escherichia coli TOP10 and E. coli LMG194 cells carrying pBADMHADgeSSB plasmid containing Deinococcus geothermalis SSB protein gene under control of the araBAD promoter. The results showed that low-level expression of T7 lysozyme did not interfere with the target SSB protein production, and that the freezing-thawing treatment was sufficient for disruption of the E. coli cells producing low amounts of T7 lysozyme.
Year
Volume
54
Issue
3
Pages
671-672
Physical description
Dates
published
2007
received
2007-08-08
revised
2007-09-10
accepted
2007-09-14
(unknown)
2007-09-17
References
  • Bollag DM, Rozycki MD, Edelstein SJ (1996) In Protein Methods, pp 27-43. Wiley-Liss Inc., New York.
  • Filipkowski P, Duraj-Thatte A, Kur J (2006) Arch Microbiol 186: 129-137.
  • Studier FW (1991) J Mol Biol 219: 37-44.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv54p671kz
Identifiers
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