The role of actin and microtubule networks in plasmid DNA intracellular trafficking

• Authors: Vladan Ondřej , Emilie Lukášová , Martin Falk , Stanislav Kozubek
• Languages of publication: EN

Abstracts

EN

This work is focused on the function of the microtubule and actin networks in plasmid DNA transport during liposomal transfection. We observed strong binding of plasmid DNA-lipid complexes (lipoplexes) to both networks and directional long-range motion of these lipoplexes along the microtubules. Disruption of either of these networks led to the cessation of plasmid transport to the nucleus, a decreased mobility of plasmids, and accumulation of plasmid DNA in large aggregates at the cell periphery. Our findings show an indispensable but different role of both types of cytoskeleton, actin and microtubular, in the processes of gene delivery.

Keywords

EN

cytoplasmic trafficking; microtubules; plasmid DNA-lipid complexes; actin filaments

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 3
• Pages: 657-663

Dates

published

2007

received

2007-05-23

revised

2007-07-04

accepted

2007-08-01

(unknown)

2007-08-23

Contributors

author

Vladan Ondřej

• Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

author

Emilie Lukášová

• Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

author

Martin Falk

• Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

author

Stanislav Kozubek

• Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

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