Menadione effect on l-cysteine desulfuration in U373 cells

• Authors: Maria Wróbel , Halina Jurkowska
• Languages of publication: EN

Abstracts

EN

The non-cytotoxic concentration (20 µM) of menadione (2-methyl-1,4-naphthoquinone), after 1 h of incubation, leads to loss of the activity of rhodanese by 33%, 3-mercaptopyruvate sulfurtransferase by 20%, as well as the level of sulfane sulfur by about 23% and glutathione by 12%, in the culture of U373 cells, in comparison with the control culture. Reactive oxygen species generated by menadione oxidize sulfhydryl groups in active centers of the investigated enzymes, inhibiting them and saving cysteine for glutathione synthesis. A decreased sulfane sulfur level can be correlated with an oxidative stress.

Keywords

EN

menadione; astrocytoma U373; sulfane sulfur; 3-mercaptopyruvate sulfurtransferase; reactive oxygen species; rhodanese

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 2
• Pages: 407-411

Dates

published

2007

received

2007-03-13

revised

2007-03-30

accepted

2007-04-16

(unknown)

2007-05-23

Contributors

author

Maria Wróbel

• Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków, Poland

author

Halina Jurkowska

• Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków, Poland

References

• Beinert H (2000) FEBS J 267: 5657-5664.
• Cannella C, Berni R (1983) FEBS Lett 162: 180-184.
• Chen Q, Cederbaum AI (1997) Mol Pharmacol 52: 648-657.
• Claiborne A, Joanne I, Mallett TC, Luba J, Crane EJ, Charrier V, Parsonnage D (1999) Biochemistry 38: 15407-15416.
• DiMonte D, Bellomo H, Thor G, Nicotera P, Orrenius S (1984a) Arch Biochem Biophys 235: 343-350.
• DiMonte D, Ross D, Bellomo G, Eklöw L, Orrenius S (1984b) Arch Biochem Biophys 235: 334-342.
• Jurkowska H, Wróbel M (2007) Amino Acids DOI: 10.1007/s00726-007-0471-2.
• Lee SR, Kwon KS, Kim SR, Rhee SG (1998) J Biol Chem 273: 15366-15372.
• Liew YF, Shaw NS (2005) J Nutr 135: 2151-2158.
• Liu B, Rotenberg SA, Mirkin MV (2000) Proc Natl Acad Sci USA 97: 9855-9860.
• Lowry OH, Rosenbrough NJ, Farr AL, Randall RI (1951) J Biol Chem 193: 265-275.
• Mauzeroll J, Bard AJ (2004) Proc Natl Acad Sci 101: 7862-7867.
• Mauzeroll J, Bard AJ, Owhadian O, Monks TJ (2004) Proc Natl Acad Sci 101: 17582-17587.
• Nagahara N, Nishino T (1996) J Biol Chem 271: 27395-27401.
• Nagahara N, Katayama A (2005) J Biol Chem 280: 34569-34576.
• Nagahara N, Okazaki T, Nishino T (1995) J Biol Chem 270: 16230-16235.
• Nandi DL, Horowitz PM, Westley J (2000) Int J Biochem Cell Biol 32: 465-473.
• Niemczyk E, Majczak A, Hallmann A, Kędzior Wodniak, Woźniak M, Wakabayashi Y (2004) Acta Biochim Polon 51: 1015-1022.
• Ogasawara Y, Isoda S, Tanabe S (1999) Biol Pharm Bull 22: 441-445.
• Ochi T (1996) Toxicology 112: 45-55.
• Ponten J, Macintyre EH (1968) Acta Pathol Microbiol Scand 74: 465-86.
• Poole LB, Claiborne A (1989) J Biol Chem 264: 12330-12338.
• Shi MM, Iwamoto T, Forman HJ (1994) Am J Physiol 267: L414-L421.
• Skorey K, Ly HD, Kelly J, Hammond M, Ramachandran C, Huang Z, Gresser MJ, Wang Q (1997) J Biol Chem 272: 22472-22480.
• Sőrbo B (1955) Methods Enzymol 2: 334-337.
• Tietze F (1969) Anal Biochem 27: 502-522.
• Valentine WN, Frankenfeld JK (1974) Clin Chim Acta 51: 205-210.
• Wefers H, Sies H (1983) Arch Biochem Biophys 224: 568-578.
• Westley J (1973) Adv Enzymol Relat Area Mol Biol 39: 327-368.
• Westley J (1980) In Enzymatic Basis of Detoxification II (Jakoby WB, ed) pp 245-262. Academic Press, New York
• Westley J, Adler H, Westley L, Nishida C (1983) Fundam Appl Toxicol 3: 377-382.
• Wood IL (1987) Methods Enzymol 43: 25-29.
• Wróbel M, Ubuka T, Yao WB, Abe T (1997) Physiol Chem Phys Med NMR 29: 11-14.
• Wróbel M, Jurkowska H, Śliwa L, Srebro Z (2004) Toxicol Mech Meth 14: 331-337.