PL EN


Preferences help
enabled [disable] Abstract
Number of results
2007 | 54 | 2 | 315-322
Article title

Thiamine deficiency caused by thiamine antagonists triggers upregulation of apoptosis inducing factor gene expression and leads to caspase 3-mediated apoptosis in neuronally differentiated rat PC-12 cells

Content
Title variants
Languages of publication
EN
Abstracts
EN
Recent evidence suggests that alterations in oxidative metabolism induced by thiamine deficiency lead to neuronal cell death. However, the molecular mechanisms underlying this process are still under extensive investigation. Here, we report that rat pheochromocytoma PC-12 cells differentiated in the presence of NGF into neurons undergo apoptosis due to thiamine deficiency caused by antagonists of thiamine - amprolium, pyrithiamine and oxythiamine. Confocal laser scanning fluorescence microscopy revealed that annexin V binds to PC-12 cells in presence of thiamine antagonists after 72 h incubation. Results also show that thiamine antagonists trigger upregulation of gene expression of mitochondrial-derived apoptosis inducing factor, DNA fragmentation, cleavage of caspase 3 and translocation of active product to the nucleus. We therefore propose that apoptosis induced by amprolium, pyrithiamine or oxythiamine occurs via the mitochondria-dependent caspase 3-mediated signaling pathway. In addition, our data indicate that pyrithiamine and oxythiamine are more potent inducers of apoptosis than amprolium.
Publisher

Year
Volume
54
Issue
2
Pages
315-322
Physical description
Dates
published
2007
received
2006-11-27
revised
2007-03-20
accepted
2007-04-17
(unknown)
2007-05-15
Contributors
  • Department of Coenzyme Biochemistry, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
  • Department of Coenzyme Biochemistry, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
  • Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, San Juan, Puerto Rico
References
  • Ali BH (1985) Effect of some monoamine oxidase inhibitors on the thiamin status of rabbits. Br J Pharmacol 86: 869-875.
  • Bakker SJ, Yin M, Kootstra G (1996) Tissue thiamine and carnitine deficiency as a possible cause of acute tubular necrosis after renal transplantation. Transplant Proc 28: 314-315.
  • Bettendorff L, Sluse F, Goessens G, Wins P, Grisar T (1995) Thiamine deficiency-induced partial necrosis and mitochondrial uncoupling in neuroblastoma cells are rapidly reversed by addition of thiamine. J Neurochem 65: 2178-2184.
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
  • Butterworth RF, Heroux M (1989) Effects of thiamine deficiency on brain metabolism: implications for the pathogenesis of the Wernicke-Korsakoff syndrome. Alcohol Alcohol 24: 271-279.
  • Calingasan NY, Chun WJ, Park LC, Uchida K, Gibson GE (1999) Oxidative stress is associated with region-specific neuronal death during thiamine deficiency. J Neuropathol Exp Neurol 58: 946-958.
  • Chornyy SA, Parkhomenko YM (2006) Activation of apoptosis in astroglial DTNC cells during metabolic disturbance caused by thiamine antagonists. Biopolymers and Cell 22: 290-298.
  • Chernikevich IP, Gritsenko EA, Lisitskaia IM, Luchko TA (1995) On vitamin B1 metabolism in avitaminosis and its correction with thiamine and taurine. Vopr Med Khim 41: 36-42.
  • Cotman CW, Anderson AJ (1995) A potential role for apoptosis in neurodegeneration and Alzheimer's disease. Mol Neurobiol 10: 19-45.
  • Chinopoulos C, Adam-Vizi V (2006) Calcium, mitochondria and oxidative stress in neuronal pathology: Novel aspects of an enduring theme. FEBS J 273: 433-450.
  • Daugas E, Susin SA, Zamzami N, Ferri KF, Irinopoulou T, Larochette N, Prevost MC, Leber B, Andrews D, Penninger J, Kroemer G (2000) Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. Faseb J 14: 729-739.
  • Desjardins P, Butterworth R (2005) Role of mitochondrial dysfunction and oxidative stress in the pathogenesis of selective neuronal loss in Wernicke's encephalopathy. Mol Neurobiol 31: 17-25.
  • Hazell AS, McGahan L, Tetzlaff W, Bedard AM, Robertson GS, Nakabeppu Y, Hakim AM (1998) Immediate-early gene expression in the brain of the thiamine-deficient rat. J Mol Neurosci 10: 1-15.
  • Heroux M, Raghavendra Rao VL, Lavoie J, Richardson JS, Butterworth RF (1996) Alterations of thiamine phosphorylation and of thiamine-dependent enzymes in Alzheimer's disease. Metab Brain Dis 11: 81-88.
  • Kruse M, Navarro D, Desjardins P, Butterworth RF (2004) Increased brain endothelial nitric oxide synthase expression in thiamine deficiency: relationship to selective vulnerability. Neurochem Int 45: 49-56.
  • Linnik MD, Zobrist RH, Hatfield MD (1993) Evidence supporting a role for programmed cell death in focal cerebral ischemia in rats. Stroke 24: 2002-2009.
  • Liu JY, Timm DE, Hurley TD (2006) Pyrithiamine as a substrate for thiamine pyrophosphokinase. J Biol Chem 281: 6601-6607.
  • Lonsdale D (2006) A review of the biochemistry, metabolism and clinical benefits of thiamin(e) and its derivatives. Evid Based Complement Alternat Med 3: 49-59.
  • Lumeng L, Edmondson JW, Schenker S, Li TK (1979) Transport and metabolism of thiamin in isolated rat hepatocytes. J Biol Chem 254: 7265-7268.
  • Martin P, Singleton CK, Hiller-Sturmhöfel S (2003) The role of thiamine deficiency in alcoholic brain disease. Alcohol Res Health 27: 134-142.
  • Oliveira FA, Galan DT, Ribeiro AM, Santos Cruz J (2007) Thiamine deficiency during pregnancy leads to cerebellar neuronal death in rat offspring: Role of voltage-dependent K(+) channels. Brain Res 1134: 79-86.
  • Pannunzio P, Hazell AS, Pannunzio M, Rao KV, Butterworth RF (2000) Thiamine deficiency results in metabolic acidosis and energy failure in cerebellar granule cells: an in vitro model for the study of cell death mechanisms in Wernicke's encephalopathy. J Neurosci Res 62: 286-292.
  • Park LC, Calingasan NY, Uchida K, Zhang H, Gibson GE (2000) Metabolic impairment elicits brain cell type-selective changes in oxidative stress and cell death in culture. J Neurochem 74: 114-124.
  • Porter AG, Urbano AG (2006) Does apoptosis-inducing factor (AIF) have both life and death functions in cells? Bioessays 28: 834-843.
  • Rais B, Comin B, Puigjaner J, Brandes JL, Creppy E, Saboureau D, Ennamany R, Lee WN, Boros LG, Cascante M (1999) Oxythiamine and dehydroepiandrosterone induce a G1 phase cycle arrest in Ehrlich's tumor cells through inhibition of the pentose cycle. FEBS Lett 456: 113-118.
  • Rindi G, Patrini C, Nauti A, Bellazzi R, Magni P (2003) Three thiamine analogues differently alter thiamine transport and metabolism in nervous tissue: an in vivo kinetic study using rats. Metab Brain Dis 18: 245-263.
  • Romanski SA, McMahon MM (1999) Metabolic acidosis and thiamine deficiency. Mayo Clin Proc 74: 259-263.
  • Rucker-Martin C, Henaff M, Hatem SN, Delpy E, Mercadier JJ (1999) Early redistribution of plasma membrane phosphatidylserine during apoptosis of adult rat ventricular myocytes in vitro. Basic Res Cardiol 94: 171-179.
  • Schon EA, Manfredi G (2003) Neuronal degeneration and mitochondrial dysfunction. J Clin Invest 111: 303-312.
  • Singleton CK, Martin PR (2001) Molecular mechanisms of thiamine utilization. Curr Mol Med 1: 197-207.
  • Stuart MC, Reutelingsperger CP, Frederik PM (1998) Binding of annexin V to bilayers with various phospholipid compositions using glass beads in a flow cytometer. Cytometry 33: 414-419.
  • Susin SA, Lorenzo HK, Zamzami N, Marzo I, Snow BE, Brothers GM, Mangion J, Jacotot E, Costantini P, Loeffler M, Larochette N, Goodlett DR, Aebersold R, Siderovski DP, Penninger JM, Kroemer G (1999) Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397: 441-446.
  • Thornberry NA, Lazebnik Y (1998) Caspases: enemies within. Science 281: 1312-1316.
  • Todd K, Butterworth RF (1999) Mechanisms of selective neuronal cell death due to thiamine deficiency. Ann N Y Acad Sci 893: 404-411.
  • Tylicki A, Lempicka A, Romaniuk-Demonchaux K, Czerniecki J, Dobrzyn P, Strumilo S (2003) Effect of oxythiamin on growth rate, survival ability and pyruvate decarboxylase activity in Saccharomyces cerevisiae. J Basic Microbiol 43: 522-529.
  • Wang JJ, Hua Z, Fentress HM, Singleton CK (2000) JNK1 is inactivated during thiamine deficiency-induced apoptosis in human neuroblastoma cells. J Nutr Biochem 11: 208-215.
  • Wang X, Wang B, Fan Z, Shi X, Ke ZJ, Luo J (2007) Thiamine deficiency induces endoplasmic reticulum stress in neurons. Neuroscience 144: 1045-1056.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv54p315kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.