Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9

• Authors: Shen Liu , Chaoqun Huang , Dawei Li , Weihua Ren , Haoxing Zhang , Meiyan Qi , Xin Li , Long Yu
• Languages of publication: EN

Abstracts

EN

We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

Keywords

EN

liver; clone; short-chain dehydrogenase/reductase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 1
• Pages: 213-218

Dates

published

2007

received

2006-03-07

revised

2006-05-30

accepted

2007-01-10

(unknown)

2007-02-20

Contributors

author

Shen Liu

• The Institute of Genetics, Fudan University, Shanghai, People's Republic of China

author

Chaoqun Huang

• Department of Biochemistry and Molecular Biology, Soochow University, Suzhou, People's Republic of China, Shanghai, People's Republic of China

author

Dawei Li

• The Institute of Genetics, Fudan University, Shanghai, People's Republic of China

author

Weihua Ren

• Guiyang Medical College, Guiyang, People's Republic of China

author

Haoxing Zhang

• The Institute of Genetics, Fudan University, Shanghai, People's Republic of China

author

Meiyan Qi

• The Institute of Genetics, Fudan University, Shanghai, People's Republic of China

author

Xin Li

• The Institute of Genetics, Fudan University, Shanghai, People's Republic of China

author

Long Yu

• The Institute of Genetics, Fudan University, Shanghai, People's Republic of China

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