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2007 | 54 | 1 | 167-174
Article title

The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria

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Abstracts
EN
The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live Gram-positive bacteria Micrococcus lysodeikticus and Gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than Gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.
Publisher

Year
Volume
54
Issue
1
Pages
167-174
Physical description
Dates
published
2007
received
2006-09-14
revised
2006-12-11
accepted
2007-01-17
(unknown)
2007-02-20
Contributors
  • Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Skłodowska University, Lublin, Poland
  • Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Skłodowska University, Lublin, Poland
  • Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Skłodowska University, Lublin, Poland
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv54p167kz
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