A novel tetrameric lectin from Lycoris aurea with four mannose binding sites per monomer

• Authors: Jiwei Liu , Xiaochao Xu , Jinzhi Liu , Jan Balzarini , Yongtin Luo , Yang Kong , Jian Li , Fang Chen , Els Van Damme , Jinku Bao
• Languages of publication: EN

Abstracts

EN

The mannose-binding agglutinin from bulbs of Lycoris aurea (LAA) agglutinates rabbit but not human erythrocytes. The molecular mass of the monomer in SDS/PAGE is 12 kDa while the apparent molecular mass in gel filtration is 48 kDa, indicating that LAA is a homotetramer. The full-length cDNA of LAA contains 683 bp with an open reading frame encoding a protomer of 162 amino-acid residues. Hydrophobic Cluster Analysis and molecular modeling of the 109-residue mature polypeptide suggested a similar secondary and tertiary structure to those of Narcissus pseudonarcissus agglutinin (NPA). Molecular docking revealed that, besides the three mannose-binding sites common among Amaryllidaceae lectins, LAA also contains a fourth unique mannose-binding site formed by a tryptophan cluster. The existence of four mannose-binding sites in each monomer of LAA is very unusual and has only been reported for NPA earlier.

Keywords

EN

sequence alignment; mannose-binding lectin; Lycoris aurea agglutinin; molecular modeling and docking

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 1
• Pages: 159-166

Dates

published

2007

received

2006-11-27

revised

2007-02-20

accepted

2007-03-05

(unknown)

2007-03-14

Contributors

author

Jiwei Liu

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Xiaochao Xu

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Jinzhi Liu

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Jan Balzarini

• Rega Institute for Medical Research, K. U. Leuven, Belgium

author

Yongtin Luo

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Yang Kong

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Jian Li

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Fang Chen

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

author

Els Van Damme

• Department of Molecular Biotechnology, Ghent University, Gent, Belgium

author

Jinku Bao

• College of Life Sciences, Sichuan University, Chengdu, P.R.China

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