Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment

• Authors: Agnieszka K Szczepańska , Monika S Hejnowicz , Piotr Kołakowski , Jacek Bardowski
• Languages of publication: EN

Abstracts

EN

We present here the results of an exploration of the bacteriophage content of dairy wheys collected from milk plants localized in various regions of Poland. Thirty-three whey samples from 17 regions were analyzed and found to contain phages active against L. lactis strains. High phage titer in all whey samples suggested phage-induced lysis to be the main cause of fermentation failures. In total, over 220 isolated phages were examined for their restriction patterns, genome sizes, genetic groups of DNA homology, and host ranges. Based on DNA digestions the identified phages were classified into 34 distinct DNA restriction groups. Phage genome sizes were estimated at 14-35 kb. Multiplex PCR analysis established that the studied phages belong to two out of the three main lactococcal phage types - c2 and 936, while P335-type phages were not detected. Yet, analyses of bacterial starter strains revealed that the majority of them are lysogenic and carry prophages of P335-type in their chromosome. Phage geographical distribution and host range are additionally discussed.

Keywords

EN

bacteriophages; dairy environment; Lactococcus lactis; biodiversity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 1
• Pages: 151-158

Dates

published

2007

received

2006-09-25

revised

2007-01-15

accepted

2007-02-07

(unknown)

2007-03-09

Contributors

author

Agnieszka K Szczepańska

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Monika S Hejnowicz

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Piotr Kołakowski

• Danisco Biolacta, Innovation, Olsztyn, Poland

author

Jacek Bardowski

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

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