Congo red interaction with α-proteins

• Authors: Jolanta Sereikaite , Vladas-Algirdas Bumelis
• Languages of publication: EN

Abstracts

EN

The ability of Congo red to form complexes with α-proteins, human growth hormone and human interferon-α2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).

Keywords

EN

dye binding; native α-proteins; Congo red; oligomerization

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 1
• Pages: 87-92

Dates

published

2006

received

2005-11-06

revised

2006-03-07

accepted

2006-03-12

Contributors

author

Jolanta Sereikaite

• Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius, Lithuania

author

Vladas-Algirdas Bumelis

• Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius, Lithuania

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