Synthetic peptides mimicking antigenic epitope of Helicobacter pylori urease

• Authors: Magdalena Białek , Sebastian Grabowski , Zbigniew Kamiński , Wiesław Kaca
• Languages of publication: EN

Abstracts

EN

Short peptides resembling the Helicobacter pylori urease antigen (UreB F8 Ser-Ile-Lys-Glu-Asp-Val-Gln-Phe) with deleted aspartic acid and glutamic acid residues, anchored through a triazine linker via the N-terminal moiety to cellulose plate were prepared. The peptides were used for binding of antibodies from sera of patients with medically confirmed atherosclerosis. Recognition of the peptides was also tested with anti-Jack beans urease antibodies. The important role of a Gly-Gly spacer separating the peptides from the cellulose support was shown. Different patterns of binding of antibodies from H. pylori infected patients and anti-Jack bean urease antibodies were observed only in the case of pentapeptides. The peptide Gly-Gly-Leu-Val-Phe-Lys-Thr was recognized by most of the tested sera.

Keywords

EN

synthetic peptides; atherosclerosis; urease epitopes; Helicobacter pylori

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 1
• Pages: 83-86

Dates

published

2006

received

2005-11-06

revised

2006-01-26

accepted

2006-02-20

(unknown)

2006-02-23

Contributors

author

Magdalena Białek

• Technical University, Department of Organic Chemistry, Łódź, Poland

author

Sebastian Grabowski

• Institute of Microbiology and Immunology, University of Łódź, Łódź, Poland

author

Zbigniew Kamiński

• Technical University, Department of Organic Chemistry, Łódź, Poland

author

Wiesław Kaca

• Institute of Biology, Swietokrzyska Academy, Kielce, Poland

References

• Deffbner M, Deimer KH, Jaeger E, Stelzel P, Thamm P, Wendlberger G, Wunsch E (1974) In Methoden der Organischen Chemie (Houben - Weyl), Synthese von Peptiden vol 15/1, pp 315-327, Georg Thieme Verlag, Stuttgart.
• Farris AD, Keech CL, Gordon TP, McCluskey J (2000) Epitope mimics and determinant spreading: pathways to autoimmunity. Cell Mol Life Sci 57: 569-578.
• Hirota K, Nagata K, Norose Y, Futagami S, Nakagawa Y, Senpuku H, Kobayashi F, Takahashi H (2001) Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production. Infect Immun 69: 6597-6603.
• Kaminski ZJ (1987) 2-Chloro-4,6-dimethoxy-1,3,5-triazine. A new coupling reagent for peptide synthesis. Synthesis 917-920.
• Kaminski ZJ, Kolesinska B, Cierpucha M (2000) The method of manufacturing of coupling reagent immobilized on the solid matrix. Pat Appl no. P-338931 from 08. 03. 2000.
• Leal-Herrera Y, Torres J, Perez G, Gomez A, Monath T, Tapia-Conyer R, Munoz O (1999) Serologic IgG response to urease in Helicobacter pylori-infected persons from Mexico. Am J Trop Med Hyg 60: 587-592.
• Ludewig B, Krebs P, Scandella E (2004) Immunopathogenesis of atherosclerosis. J Leukocyte Biol 76: 300-306.
• Mendall M (1994) Relation of Helicobacter pylori infection and coronary heart disease. Br Heart J 71: 437-439.
• Rechcinski T, Grebowska A, Kurpesa M, Rudnicka W, Krzeminska-Pakuła M, Chmiela M (2005) Occurrence of serum class G immunoglobulins interacting with specific antigens of Helicobacter pylori in patients with unstable coronary artery disease and in symptomless individuals. Pol J Microbiol 54: 221-225.
• Wucherpfennig KW (2001) Structural basis of molecular mimicry. J Autoimmun 16: 293-302.