Effect of Rho-associated kinase inhibition on actin cytoskeleton structure and calcium response in glioma C6 cells

• Authors: Berenika Targos , Paweł Pomorski , Patryk Krzemiński , Jolanta Barańska , Maria Jolanta Rędowicz , Wanda Kłopocka
• Languages of publication: EN

Abstracts

EN

The role of actin cytoskeleton functional state in glioma C6 cell morphology and calcium signaling was investigated through modification of myosin II activity by blocking Rho-associated kinase with the specific inhibitor Y-27632. Treatment of glioma C6 cells with ROCK inhibitor resulted in actin cytoskeleton reorganization and also in the changed shape and distribution of mitochondria. Changes in the distribution of ER, the main calcium store in glioma C6 cells, were not visible. The inhibition of myosin II activity influences the first phase of calcium signaling evoked by agonist, and both phases of thapsigargin-evoked calcium response. We suggest that the observed increase in Ca2+ release from intracellular stores induced by IP3 formation as well as inhibition of SERCA ATPase is at least in part related to severely affected mitochondria. Enhancement of capacitative calcium entry evoked by thapsigargin is probably associated with the reorganization of the acto-myosin II system. ATP-induced calcium response presents no changes in the second phase. We observed that ATP stimulation of Y-27632 pretreated cells leads to immediate morphological rearrangement of glioma C6 cells. It is a consequence of actin cytoskeleton reorganization: formation of stress fibers and relocation of phosphorylated myosin II to actin filaments. It seems that the agonist-evoked strong calcium signal may be sufficient for myosin II activation and the stress fiber organization. This is the first work showing the dependence between the functional state of the acto-myosin II system and calcium signaling stressing the reversible character of this relationship.

Keywords

EN

glioma C6; actin cytoskeleton; RhoA; ROCK; calcium transients

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 4
• Pages: 825-831

Dates

published

2006

received

2006-10-01

revised

2006-11-08

accepted

2006-11-16

(unknown)

2006-11-19

Contributors

author

Berenika Targos

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warszawa, Poland

author

Paweł Pomorski

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warszawa, Poland

author

Patryk Krzemiński

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warszawa, Poland

author

Jolanta Barańska

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warszawa, Poland

author

Maria Jolanta Rędowicz

• Department of Muscle Biochemistry, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warszawa, Poland

author

Wanda Kłopocka

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warszawa, Poland

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