Activity of paraoxonase 1 (PON1) and its relationship to markers of lipoprotein oxidation in healthy Slovaks

• Authors: Katarína Sumegová , Pavel Blažíček , Iveta Waczulíková , Ingrid Žitňanová , Zdeňka Ďuračková
• Languages of publication: EN

Abstracts

EN

Low-density lipoproteins (LDLs), when modified by free radicals derived from artery wall cells, induce atherosclerosis. In contrast to oxidized LDL (ox-LDL), high-density lipoproteins (HDLs) are able to prevent atherosclerosis through a protein with antioxidant properties, paraoxonase 1 (PON1). The purpose of this study was to explore the association between the activity of HDL-associated PON1 and circulating ox-LDL as well as to investigate the relationship between ox-LDL and parameters of lipid profile in thirty Slovaks aged 21-73 years because recent studies have presented controversial results concerning PON1 and its role in LDL oxidation. For determination of circulating ox-LDL sandwich ELISA was used and other lipid parameters were determined by routine laboratory analyses. PON1 activities were assayed by two synthetic substrates - paraoxon and phenyl acetate. Lipid peroxides were determined spectrophotometrically. Of the lipid parameters examined, ox-LDL level correlated positively with total (P < 0.0001) and LDL-cholesterol (P < 0.001). Triacylglycerols (TAG) (P < 0.001), lipid peroxides (P < 0.01) and atherogenic index (AI = total cholesterol/HDL) (P < 0.0001) were also strongly correlated with ox-LDL. No inverse relationships were observed between ox-LDL and HDL-cholesterol or arylesterase/paraoxonase activities of PON1. Furthermore, it was found that ox-LDL (P < 0.01) and lipid peroxides (P < 0.05) were significantly higher in men than in women. PON1 arylesterase activity was marginally affected by sex. The results of this study suggest that the anti-atherogenic properties of HDLs are not directly related to their total concentration and that PON1 activity determined towards synthetic compounds (paraoxon and phenyl acetate) reflects no association with markers of oxidative stress. Furthermore, it follows from our results that men are more susceptible to developing atherosclerosis compared to women.

Keywords

EN

paraoxonase 1; atherosclerosis; ox-LDL; HDL

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 4
• Pages: 783-787

Dates

published

2006

received

2006-03-17

revised

2006-10-05

accepted

2006-10-16

(unknown)

2006-11-14

Contributors

author

Katarína Sumegová

• Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, School of Medicine, Comenius University, Bratislava, Slovakia

author

Pavel Blažíček

• Division of Biochemical Clinical Laboratories, Hospital of Ministry of Defence, Bratislava, Slovakia

author

Iveta Waczulíková

• Department of Nuclear Physics and Biophysics, Division of Biomedical Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia

author

Ingrid Žitňanová

• Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, School of Medicine, Comenius University, Bratislava, Slovakia

author

Zdeňka Ďuračková

• Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, School of Medicine, Comenius University, Bratislava, Slovakia

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