A novel Δ12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115

• Authors: D SH Wei , M CH Li , X X Zhang , H Zhou , L J Xing
• Languages of publication: EN

Abstracts

EN

The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Δ12 and Δ15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Δ12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Δ12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Δ15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Δ12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Δ12-fatty acid desaturase gene in methylotrophic yeast.

Keywords

EN

fatty acid desaturase gene; Pichia pastoris; Saccharomyces cerevisiae; linoleic acid

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 4
• Pages: 753-759

Dates

published

2006

received

2006-03-10

revised

2006-08-09

accepted

2006-10-04

(unknown)

2006-10-26

Contributors

author

D SH Wei

• Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China

author

M CH Li

• Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China

author

X X Zhang

• Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China

author

H Zhou

• Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China

author

L J Xing

• Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China

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