The YJL185C, YLR376C and YJR129C genes of Saccharomyces cerevisiae are probably involved in regulation of the glyoxylate cycle

• Authors: Ewa Boniewska-Bernacka , Robert Wysocki , Renata Grochowalska , Beata Machnicka , Stanisław Ułaszewski , Tadeusz Lachowicz
• Languages of publication: EN

Abstracts

EN

The ER24 aci (acidification) mutant of Saccharomyces cerevisiae excreting protons in the absence of glucose was transformed with a multicopy yeast DNA plasmid library. Three different DNA fragments restored the wild-type phenotype termed Aci- because it does not acidify the complete glucose medium under the tested conditions. Molecular dissection of the transforming DNA fragments identified two multicopy suppressor genes YJL185C, YJR129C and one allelic YLR376C. Disruption of either of the three genes in wild-type yeast strain resulted in acidification of the medium (Aci+ phenotype) similarly to the original ER24 mutant. These data indicate the contribution of the ER24 gene product Ylr376Cp and of the two suppressor gene products Yjl185Cp and Yjr129Cp to a complex regulation of the glyoxylate cycle in yeast.

Keywords

EN

glyoxylate cycle; multicopy suppressor; Saccharomyces cerevisiae; acidification mutants; yeast

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 4
• Pages: 739-745

Dates

published

2006

received

2006-02-20

revised

2006-10-16

accepted

2006-10-18

(unknown)

2006-12-04

Contributors

author

Ewa Boniewska-Bernacka

• Department of Biotechnology and Molecular Biology, University of Opole, Opole, Poland

author

Robert Wysocki

• Institute of Genetics and Microbiology, Wroclaw University, Wrocław, Poland

author

Renata Grochowalska

• Institute of Biotechnology and Environmental Protection, University of Zielona Góra, Zielona Góra, Poland

author

Beata Machnicka

• Institute of Biotechnology and Environmental Protection, University of Zielona Góra, Zielona Góra, Poland

author

Stanisław Ułaszewski

• Institute of Genetics and Microbiology, Wroclaw University, Wrocław, Poland

author

Tadeusz Lachowicz

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