IS1 transposition is enhanced by translation errors and by bacterial growth at extreme glucose levels

• Authors: Arun S Kharat , Evelyne Coursange , Marjolaine Noirclerc-Savoye , Jérôme Lacoste , Michel Blot
• Languages of publication: EN

Abstracts

EN

Transposition of insertion sequences (IS) is an enzyme-mediated process that only occurs in a minority of cells within a bacterial culture. Transposition is thus a rare event, but transposition frequency may vary depending on experimental conditions. For instance in a rich broth, IS elements are known to transpose during stationary phase but not during exponential growth. Using a reporter system which involves the activation of the cryptic bgl operon in Escherichia coli, we show that the frequency of IS1 transposition is a function of glucose concentration in the growth medium, it is increased by streptomycin amounts that are below minimum inhibitory concentration (sub-MIC) and is inhibited in an rpsL150 strain with high translation accuracy. Since starved cells are known to enhance ribosome frameshifting, our data suggests that growth conditions applied in this study could affect IS1 transposition by increasing translation infidelity.

Keywords

EN

programmed translation frameshift; β-glucosides; bgl operon; IS elements

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 4
• Pages: 729-738

Dates

published

2006

received

2006-04-12

revised

2006-08-16

accepted

2006-10-04

(unknown)

2006-11-14

Contributors

author

Arun S Kharat

• Plasticité et Expression des Génomes Microbiens, CNRS-FRE2383/CEA LRC12/Université Joseph Fourier, Grenoble, France

author

Evelyne Coursange

• Plasticité et Expression des Génomes Microbiens, CNRS-FRE2383/CEA LRC12/Université Joseph Fourier, Grenoble, France

author

Marjolaine Noirclerc-Savoye

• Plasticité et Expression des Génomes Microbiens, CNRS-FRE2383/CEA LRC12/Université Joseph Fourier, Grenoble, France

author

Jérôme Lacoste

• Plasticité et Expression des Génomes Microbiens, CNRS-FRE2383/CEA LRC12/Université Joseph Fourier, Grenoble, France

author

Michel Blot

• Plasticité et Expression des Génomes Microbiens, CNRS-FRE2383/CEA LRC12/Université Joseph Fourier, Grenoble, France

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