Assessment of various diagnostic methods of ureaplasma respiratory tract infections in newborns

• Authors: Małgorzata Biernat-Sudolska , Danuta Rojek-Zakrzewska , Ryszard Lauterbach
• Languages of publication: EN

Abstracts

EN

We compared three methods used microbial culturing for detection of ureaplasmas in endotracheal aspirate from 500 prematurely born neonates with respiratory disturbances: BioMerieux test, PCR and microbial culturing. Ureaplasmas were detected in respiratory tracts of 79 (16%) newborns. Correlation of the results of culture with those obtained with the BioMerieux kit, culture with PCR and BioMerieux kit with PCR was 97%, 89% and 90%, respectively. Sensitivity and specificity of PCR in comparison with culture was 86% and 98%, respectively, and of the BioMerieux kit 96% and 98%. PCR can be recommended in rapid diagnostics of respiratory infections in newborns suffering from respiratory disorders. It allows the detection of ureaplasmas in case of parallel infections and identification of their species.

Keywords

EN

ureaplasma; PCR; culture; BioMerieux kit; newborn

• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 609-612

Dates

published

2006

received

2006-06-06

revised

2006-08-04

accepted

2006-09-10

(unknown)

2006-10-01

Contributors

author

Małgorzata Biernat-Sudolska

• Department Virology, Chair of Microbiology, Medical College of Jagiellonian University, Kraków, Poland

author

Danuta Rojek-Zakrzewska

• Department Virology, Chair of Microbiology, Medical College of Jagiellonian University, Kraków, Poland

author

Ryszard Lauterbach

• Chair of Neonatology, Medical College of Jagiellonian University, Kraków, Poland

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