Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers

• Authors: Slawa Szostek , Malgorzata Klimek , Barbara Zawilinska , Janusz Rys , Jolanta Kope , Ewa Daszkiewic
• Languages of publication: EN

Abstracts

EN

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n = 42) and abnormal (n = 83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (χ2, P < 0.0005). The correlation between assays with the use of SPF10 and MY09/MY11 was 86% and between hybrid capture and the pU-1M/pU2R technique - 78%. In 49% of samples HPV DNA was detected by the four methods, whereas in 12% only by the SPF10 primers. The most sensitive technique was found to be PCR with the use of SPF10 primers, while the most specific - the MY09/11 PCR method. It seems that home-PCR with MY09/MY11 primers could be applied in screening tests.

Keywords

EN

hybrid capture; PCR; HPV detection; papillomavirus

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 603-607

Dates

published

2006

received

2006-06-06

revised

2006-08-31

accepted

2006-09-18

(unknown)

2006-10-01

Contributors

author

Slawa Szostek

• Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland

author

Malgorzata Klimek

• Centre of Oncology, M. Sklodowska-Curie Memorial Institute, Kraków, Poland

author

Barbara Zawilinska

• Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland

author

Janusz Rys

• Centre of Oncology, M. Sklodowska-Curie Memorial Institute, Kraków, Poland

author

Jolanta Kope

• Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland

author

Ewa Daszkiewic

• Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland

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