Preferences help
enabled [disable] Abstract
Number of results
2006 | 53 | 3 | 603-607
Article title

Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers

Title variants
Languages of publication
The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n = 42) and abnormal (n = 83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (χ2, P < 0.0005). The correlation between assays with the use of SPF10 and MY09/MY11 was 86% and between hybrid capture and the pU-1M/pU2R technique - 78%. In 49% of samples HPV DNA was detected by the four methods, whereas in 12% only by the SPF10 primers. The most sensitive technique was found to be PCR with the use of SPF10 primers, while the most specific - the MY09/11 PCR method. It seems that home-PCR with MY09/MY11 primers could be applied in screening tests.
Physical description
  • Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV (2002) J Clin Pathol 55: 244-265.
  • Cavuslu S, Mant C, Starkey WG, Bible JM, Biswas C, Kell B, Rice P, Best JM, Cason J (1996) J Med Virol 49: 319-324.
  • Clavel C, Masure M, Bory JP, Putaud I, Mangeonjean C, Lorenzato M, Gabriel R, Quereux C, Birembaut P (1999) Br J Cancer 80: 1306-1311.
  • Cox JT, Lorincz AT, Schiffman MH, Sherman ME, Cullen A, Kurman RJ (1995) Am J Obstet Gynecol 172: 946-954.
  • De Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H (2004) Virology 324: 17-27.
  • Dillner J (1999) Semin Cancer Biol 9: 423-430.
  • Fauquet CM, Mayo MA (2005) In Virus Taxonomy (Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, eds) pp 239-253, Elsevier Academic Press, London, New York.
  • Fujinaga Y Shimada M, Okazawa K, Fukushima M, Kato I, Fujinaga K (1991) J Gen Virol 72: 1039-1044.
  • Gravitt PE, Peyton CL, Alessi TQ, Wheeler CM, Coutlee F, Hildesheim A, Shiffman MH, Scott DR, Apple RJ (2000) J Clin Microbiol 38: 357-361.
  • Jacobs MV, Snijders PJ, van der Brule AJ, Helmerhorst TJ, Meijer CJ, Walboomers JM (1997) J Clin Microbiol 35: 791-795.
  • Kleter B, van Doorn L-J, ter Schegget J, Schrauwen L, van Krimpen C, Burger M, ter Harmsel B, Quint W (1998) Am J Pathol 153: 1731-1739.
  • Lorincz AT (1996) J Obstet Gynaecol Res 22: 629-636.
  • Low SH, Thong TW, Ho TH, Lee YS, Morita T, Singh M, Yap EH, Chan YC (1990) Jpn J Cancer Res 81: 1118-1123.
  • Lowy DR, Howley PM (2001) In Fields Virology (Knipe DE, Howley PM, eds) pp 2231-2264. Lippincott Williams & Wilkins, A Wolters Kluwer Company, New York, London.
  • Manos MM, Ting Y, Wright DK, Lewis AI, Broker TR, Wolinsky SM (1989) Cancer Cells 7: 209-214.
  • Meissner JD (1999) J Gen Virol 80: 1725-1733.
  • Munoz N, Bosch FX, de Sanjose S, Herrero R, Castellsague X, Shah KV, Snijders PJF, Meijer CJLM (2003) N Engl J Med 348: 518-527.
  • Perrons C, Kleter B, Jelley R, Jalal H, Quint W, Tedder R (2002) J Med Virol 67: 246-252.
  • Perrons C, Jelley R, Kleter B, Quint W, Brink N (2005) J Clin Virol 32: 278-285.
  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Science 230: 1350-1354.
  • Schmidt MT, Olejnik AK, Gozdzicka-Jozefiak A (2005) Acta Biochim Polon 52: 823-832.
  • zur Housen H (2000) J Natl Cancer Inst 92: 690-698.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.