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2006 | 53 | 3 | 597-601
Article title

Ganoderma lucidum extract stimulates glucose uptake in L6 rat skeletal muscle cells

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EN
Abstracts
EN
The effect of Ganoderma lucidum extract on glucose uptake was studied in L6 rat skeletal muscle cells. G. lucidum extract increased glucose uptake about 2-fold compared to control. The extract stimulated the activity of phosphatidylinositol (PI) 3-kinase which is a major regulatory molecule in the glucose uptake pathway. About 7-fold increased activity of a PI 3-kinase was observed after treatment with G. lucidum extract, whereas PI 3-kinase inhibitor, LY294002, blocked the G. lucidum extract-stimulated PI 3-kinase activity in L6 skeletal muscle cells. Protein kinase B, a downstream mediator of PI 3-kinase, was also activated by G. lucidum extract. We then assessed the activity of AMP-activated protein kinase (AMPK), another regulatory molecule in the glucose uptake pathway. G. lucidum extract increased the phosphorylation level of both AMPK α1 and α2. Activity of p38 MAPK, a downstream mediator of AMPK, was also increased by G. lucidum extract. Taken together, these results suggest that G. lucidum extract may stimulate glucose uptake, through both PI 3-kinase and AMPK in L6 skeletal muscle cells thereby contributing to glucose homeostasis.
Publisher

Year
Volume
53
Issue
3
Pages
597-601
Physical description
Dates
published
2006
received
2006-03-29
revised
2006-08-18
accepted
2006-09-08
(unknown)
2006-09-10
Contributors
  • Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
author
  • Department of Biochemistry, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
author
  • Department of Obesity Science, Dongduk Women's University, Seoul, Republic of Korea
  • Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
author
  • Department of Food and Biotechnology, Hanseo University, Seosan, Republic of Korea, Seoul, Republic of Korea
  • Division of Rheumatology, Department of Internal Medicine, Kyung Hee Medical Center, Seoul, Republic of Korea
author
  • Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
author
  • Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p597kz
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