Tocopherol esters inhibit human glutathione S-transferase omega

• Authors: Adriana Sampayo-Reyes , Robert A Zakharyan
• Languages of publication: EN

Abstracts

EN

Human glutathione S-transferase omega 1-1 (hGSTO1-1) is a newly identified member of the glutathione S-transferase (GST) family of genes, which also contains alpha, mu, pi, sigma, theta, and zeta members. hGSTO1-1 catalyzes the reduction of arsenate, monomethylarsenate (MMA(V)), and dimethylarsenate (DMA(V)) and exhibits thioltransferase and dehydroascorbate reductase activities. Recent evidence has show that cytokine release inhibitory drugs, which specifically inhibit interleukin-1b (IL-1b), directly target hGSTO1-1. We found that (+)-α-tocopherol phosphate and (+)-α-tocopherol succinate inhibit hGSTO1-1 in a concentration-dependent manner with IC50 values of 2 µM and 4 µM, respectively. A Lineweaver-Burk plot demonstrated the uncompetitive nature of this inhibition. The molecular mechanism behind the inhibition of hGSTO1-1 by α-tocopherol esters (vitamin E) is important for understanding neurodegenerative diseases, which are also influenced by vitamin E.

Keywords

EN

glutathione S-transferase; human; hGSTO1-1; MMA(V) reductase; Vitamin E

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 547-552

Dates

published

2006

received

2006-02-02

revised

2006-06-23

accepted

2006-08-09

(unknown)

2006-10-01

Contributors

author

Adriana Sampayo-Reyes

• División de Farmacología y Toxicología, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México

author

Robert A Zakharyan

• Department of Molecular and Cellular Biology; The University of Arizona, Tucson, Arizona, USA

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