Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L
Languages of publication
Bim is a pro-apoptotic member of the Bcl-2 protein family. Overexpression of Bim proved to be highly cytotoxic for diverse cells.The AD293 cell line is derived directly from the HEK293 cell line but has been transfected with a gene that can improve cell adherence.We found that there was almost no apoptosis seen in Bim L-transfected AD293 cells, but more than half ofBim L-transfected HEK293 cells underwent apoptosis. Suppression subtractive hybridizationwas used to detect the different gene expression profile between these two cell lines. In 192 sequencedpositive clones, there were 30 clones repeating twice or more. Ten genes were selected for identification by semi-quantitative RT-PCR.Thetranscripts of two adhesion-relatedgenes (actin and parvin)and two apoptosis-related genes (cyclin 2 and protein phosphatase 1G) were up-regulated in AD293 cells. These results suggest that the high expression of cell adhesion-related proteins might be responsible for the different apoptosis status after the transfection of Bim L.Our data provide candidate genes responsible for the different apoptosis sensitivity of these two cell lines. Further investigation on thedifferential expression profile between AD293 and HEK293 might improve our understanding of cell apoptosis mechanism.
- Adams JM, Cory S (1998) The Bcl-2 protein family: arbiters of cell survival. Science 281: 1322-1326.
- Antonsson B (2004) Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Mol Cell Biochem 256-257: 141-155.
- Bidere N, Lorenzo HK, Carmona S, Laforge M, Harper F, Dumont C, Senik A (2003) Cathepsin D triggers Bax activation, resulting in selective apoptosis-inducing factor (AIF) relocation in T lymphocytes entering the early commitment phase to apoptosis. J Biol Chem 278: 31401-31411.
- Burlacu A (2003) Regulation of apoptosis by Bcl-2 family proteins. J Cell Mol Med 7: 249-257.
- Collins NL, Reginato MJ, Paulus JK, Sgroi DC, Labaer J, Brugge JS (2005) G1/S cell cycle arrest provides anoikis resistance through Erk-mediated Bim suppression. Mol Cell Biol 25: 5282-5291.
- Endo K, Kohnoe S, Tsujita E, Watanabe A, Nakashima H, Baba H, Maehara Y (2005) Modulation of anti-apoptosis by endogenous IAP expression in MKN45 human gastric cancer cells. Anticancer Res 25: 2713-2717.
- Erba HP, Eddy R, Shows T, Kedes L, Gunning P (1988) Structure, chromosome location, and expression of the human gamma-actin gene: differential evolution, location, and expression of the cytoskeletal beta- and gamma-actin genes. Mol Cell Biol 8: 1775-1789.
- Fukazawa H, Noguchi K, Masumi A, Murakami Y, Uehara Y (2004) BimEL is an important determinant for induction of anoikis sensitivity by mitogen-activated protein/extracellular signal-regulated kinase kinase inhibitors. Mol Cancer Ther 3: 1281-1288.
- Graham FL, Smiley J, Russell WC, Nairn R (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 36: 59-74.
- Izumi H, Ise T, Murakami T, Torigoe T, Ishiguchi H, Uramoto H, Yoshida Y, Yoshida T, Tanabe M, Kohno K (2003) Structural and functional characterization of two human V-ATPase subunit gene promoters. Biochim Biophys Acta 1628: 97-104.
- JinZhong Chen, ChaoNeng Ji, ShaoHua Gu, JiXi Li, EnPeng Zhao, Huang Y, Huang L, Ying K, Xie Y, Mao YM (2004) Over-expression of Bim_3, a novel isoform of human Bim, result in cell apoptosis. Int J Biochem Cell Biol 36: 1554-1561.
- Kasof GM, Goyal L, White E (1999) Btf, a novel death-promoting transcriptional repressor that interacts with Bcl-2-related proteins. Mol Cell Biol 19: 4390-4404.
- Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis, a basic biological phenomenon with wide-ranging implications in tissue kinetics Br J Cancer 26: 239-257.
- Mami U, Miyashita T, Shikama Y, Tadokoro K, Yamada M (2001) Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family. FEBS Lett 509: 135-141.
- Marani M, Tenev T, Hancock D, Downward J, Lemoine NR (2002) Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol 22: 3577-3589.
- Monteiro HP, Silva EF, Stern A (2004) Nitric oxide: apotential inducer of adhesion-related apoptosis-anoikis. Nitric Oxide 10: 1-10.
- O'Connor L, Strusser A, O'Reilly LA, Hausman J, Adams JM, Cory S, Huang DC (1998) Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J 17: 384-395.
- Oltvai ZN, Milliman CL, Korsmeyer SJ (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619.
- O'Reilly LA, Cullen L, Visvader J, Lindeman GJ, Print C (2000) The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells. Am J Pathol 157: 449-461.
- Puthalakath H, Huang D, O'Reilly LA, King SM, Strasser A (1999) The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex. Mol Cell 3: 287-296.
- Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, Huang DC, Strasser A (2001) Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 293: 1829-1832.
- Strasser A, O'Connor L, Dixit VM (2000) Apoptosis signaling. Annu Rev Biochem 69: 217-245.
- Tanimoto T, Tsuda H, Imazeki N, Ohno Y, Imoto I, Inazawa J, Matsubara O (2005) Nuclear expression of cIAP-1, an apoptosis inhibiting protein, predicts lymph node metastasis and poor patient prognosis in head and neck squamous cell carcinomas. Cancer Lett 224: 141-151.
- Thompson CB (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267: 1456-1462.
- Vaux DL, Cory S, Adams JM (1988) Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature 335: 440-442.
- Wakako T, Tatsuhiko K, Takao H, Keiji T, Toshiaki S, Hiroyuki A, Naeko M, Noriko N (2001) Anti-atherogenic antioxidants regulate the expression and function of proteasome α-type subunits in human endothelial cells. J Biol Chem 276: 40497-40501.
- White E (1996) Life, death, and the pursuit of apoptosis. Gene Dev 10: 1-15.
Publication order reference