On the possibility that H1 histone interaction with DNA occurs through phosphates connecting lysine and arginine side chain groups

• Authors: Marina Piscopo , Luciano De Petrocellis , Mariachiara Conte , Giovanna Pulcrano , Giuseppe Geraci
• Languages of publication: EN

Abstracts

EN

Gel filtration and velocity of sedimentation analyses on native and on lysine- and arginine- modified forms of the annelid worm Chaetopterus variopedatus sperm H1 histone indicate that anion-mediated lysine - arginine interactions play a relevant role in the stabilization of the oligomeric states of the molecule. CD spectroscopy shows that phosphate anions are at least an order of magnitude more efficient than chloride as negatively charged groups connecting H1 lysines and arginines. Acetylation of lysines, although not altering grossly the H1 properties, causes a tenfold decrease of the structuring efficiency of phosphates. This suggests that DNA phosphates may be sandwiched between lysine and arginine groups of H1 histone when this molecule binds to chromatin, constituting a relevant parameter for the reciprocal stabilization of the protein and of the chromatin higher order structures.

Keywords

EN

Chaetopterus variopedatus; protein structure; sperm H1 histone; arginine; lysine; ionic interaction

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 507-513

Dates

published

2006

received

2006-06-28

revised

2006-08-31

accepted

2006-09-07

(unknown)

2006-10-01

Contributors

author

Marina Piscopo

• Department of Structural and Functional Biology, Section of Genetic and Molecular Biology, University of Naples Federico II, Naples, Italy

author

Luciano De Petrocellis

• CNR-Institute of Cybernetics "Eduardo Caianiello" Pozzuoli-Naples, Naples, Italy

author

Mariachiara Conte

• Department of Structural and Functional Biology, Section of Genetic and Molecular Biology, University of Naples Federico II, Naples, Italy

author

Giovanna Pulcrano

• Department of Structural and Functional Biology, Section of Genetic and Molecular Biology, University of Naples Federico II, Naples, Italy

author

Giuseppe Geraci

• Department of Biological Sciences, Section of Genetic and Molecular Biology; University of Naples Federico II, Naples, Italy

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