Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome

• Authors: Krzysztof Hinc , Adam Iwanicki , Simone Seror , Michał Obuchowski
• Languages of publication: EN

Abstracts

EN

The genome sequence of the Gram-positive soil bacterium Bacillus subtilis was completed in 1997 (Kunst et al., 1998) and the results included the identification of a putative transcription unit encompassing the yloI to yloS genes. Within this region of the B. subtilis chromosome 11 putative open reading frames were found with a wide diversity of probable functions. In this work we have analyzed transcription in the region of the priA-cpgA genes and we have mapped a promoter which is located inside the priA gene and its activity directs transcription of the def-yloM genes. Moreover, this transcript can be extended at low level to the prpC-priK-cpgA genes. Analysis of the sequence in proximity of the transcription start site revealed a sequence suitable for the housekeeping σA subunit of RNA polymerase. Analysis of the β-glactosidase activity of transcription fusions revealed that the identified promoter is active at low level and its activity is increased during late exponential phase of growth.

Keywords

EN

weak promoters; Bacillus subtilis; transcription

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 497-505

Dates

published

2006

received

2006-01-05

revised

2006-07-18

accepted

2006-08-04

(unknown)

2006-09-09

Contributors

author

Krzysztof Hinc

• Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

author

Adam Iwanicki

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk, Gdańsk, Poland

author

Simone Seror

• Institute of Genetics and Microbiology, University of Paris-Sud, Orsay Cedex, France

author

Michał Obuchowski

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk, Gdańsk, Poland

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