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2006 | 53 | 3 | 497-505
Article title

Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome

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EN
Abstracts
EN
The genome sequence of the Gram-positive soil bacterium Bacillus subtilis was completed in 1997 (Kunst et al., 1998) and the results included the identification of a putative transcription unit encompassing the yloI to yloS genes. Within this region of the B. subtilis chromosome 11 putative open reading frames were found with a wide diversity of probable functions. In this work we have analyzed transcription in the region of the priA-cpgA genes and we have mapped a promoter which is located inside the priA gene and its activity directs transcription of the def-yloM genes. Moreover, this transcript can be extended at low level to the prpC-priK-cpgA genes. Analysis of the sequence in proximity of the transcription start site revealed a sequence suitable for the housekeeping σA subunit of RNA polymerase. Analysis of the β-glactosidase activity of transcription fusions revealed that the identified promoter is active at low level and its activity is increased during late exponential phase of growth.
Publisher

Year
Volume
53
Issue
3
Pages
497-505
Physical description
Dates
published
2006
received
2006-01-05
revised
2006-07-18
accepted
2006-08-04
(unknown)
2006-09-09
Contributors
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
author
  • Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk, Gdańsk, Poland
author
  • Institute of Genetics and Microbiology, University of Paris-Sud, Orsay Cedex, France
  • Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk, Gdańsk, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p497kz
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