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2006 | 53 | 2 | 421-424
Article title

NMR-based localization of ions involved in salting out of hen egg white lysozyme

Content
Title variants
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EN
Abstracts
EN
NaCl-induced aggregation of hen egg white lysozyme (HEWL) was monitored by NMR spectroscopy. Small, but significant, changes induced by salt addition in TOCSY spectra were attributed to the effect of local reorganization of protein backbone upon ion binding. Salt-induced variations in HN and Hα chemical shifts were mapped on the HEWL 3D structure which allowed the construction of a scheme of the spatial localization of potential ion binding sites. It was found that in a 0.5 M NaCl solution six chloride anions and at least one sodium cation are bound to preferred sites on the HEWL surface.
Keywords
Publisher

Year
Volume
53
Issue
2
Pages
421-424
Physical description
Dates
published
2006
received
2006-03-28
revised
2006-04-11
accepted
2006-05-30
(unknown)
2006-06-12
Contributors
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p421kz
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