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2006 | 53 | 2 | 399-405
Article title

PrPd accumulation in organs of ARQ/ARQ sheep experimentally infected with BSE by peripheral routes

Content
Title variants
Languages of publication
EN
Abstracts
EN
To study the pathogenesis of bovine spongiform encephalopathy infection in small ruminants, two Lacaune sheep with the AA136RR154QQ171 and one with the AA136RR154RR171 genotype for the prion protein, were inoculated with a brain homogenate from a French cattle BSE case by peripheral routes. Sheep with the ARQ/ARQ genotype are considered as susceptible to prion diseases contrary to those with the ARR/ARR genotype. The accumulation of disease-associated prion protein (PrPd) was analysed by biochemical and immunohistochemical methods. No PrPd accumulation was detected in samples from the ARR/ARR sheep 2 years post inoculation. In the two ARQ/ARQ sheep that had scrapie-like clinical symptoms, PrPd was found in the central, sympathetic and enteric nervous systems and in lymphoid organs. Remarkably, PrPd was also detected in some muscle types as well as in all peripheral nerves that had not been reported previously thus revealing a widespread distribution of BSE-associated PrPd in sheep tissues.
Keywords
EN
Year
Volume
53
Issue
2
Pages
399-405
Physical description
Dates
published
2006
received
2006-02-01
revised
2006-05-04
accepted
2006-05-16
(unknown)
2006-06-12
References
  • Andreoletti O, Berthon P, Marc D, Sarradin P, Grosclaude J, vanKeulen L, Schelcher F, Elsen JM, Lantier F (2000) Early accumulation of PrPSc in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie. J Gen Virol 81: 3115-3126.
  • Andreoletti O, Simon S, Lacroux C, Morel N, Tabouret G, Chabert A, Lugan S, Corbiere F, Ferre P, Foucras G, Laude H, Eychenne F, Grassi J, Schelcher F (2004) PrPSc accumulation in myocytes from sheep incubating natural scrapie. Nat Med 10: 591-593.
  • Archer F, Bachelin C, Andreoletti O, Besnard N, Perrot G, Langevin C, Le Dur A, Vilette D, Van Evercooren A, Vilotte J, Laude H (2004) Cultured peripheral neuroglial cells are highly permissive to sheep prion infection. J Virol 78: 482-490.
  • Betemps D, Baron T (2001) Molecular specificities of antibodies against ovine and murine recombinant prion proteins. Biochem Biophys Res Comm 281: 101-108.
  • Biacabe AG, Laplanche JL, Ryder S, Baron T (2004) Distinct molecular phenotypes in bovine prion diseases. EMBO Reports 5: 110-115.
  • Bolton DC, McKinley MP, Prusiner SB (1982) Identification of a protein that purifies with the scrapie prion. Science 218: 1309-1311.
  • Bruce ME, Will RG, Ironside JW, McConnell I, Drummond D, Suttle A, McCardle L, Chree A, Hope J, Birkett C, Cousens S, Fraser H, Bostock CJ (1997) Transmission to mice indicate that “new variant” CJD is caused by the BSE agent. Nature 389: 498-501.
  • Buschmann A, Luhken G, Schultz J, Erhardt G, Groschup M (2004) Neuronal accumulation of abnormal prion protein in sheep carrying a scrapie-resistant genotype (PrPARR/ARR). J Gen Virol 85: 2727-2733.
  • Eloit M, Adjou K, Coulpier M, Fontaine J, Hamel R, Lilin T, Messiaen S, Andreoletti O, Baron T, Bencsik A, Biacabe A, Beringue V, Laude H, Le Dur A, Vilotte J, Comoy E, Deslys J, Grassi J, Simon S, Lantier F, Sarradin P (2005) BSE agent signatures in a goat. Vet Rec 156: 523-524.
  • Elsen J-M, Amigues Y, Schelcher F, Ducrocq V, Andréoletti O, Eychenne F, Vu Tien Khang J, Poivey J-P, Lantier F, Laplanche J-L (1999) Genetic susceptibility and transmission factors in scrapie: detailed analysis of an epidemic in a closed flock of Romanov. Arch Virol 144: 431-445.
  • Ersdal C, Ulvund M, Espenes A, Benestad S, Sarradin P, Landsverk T (2005) Mapping PrPSc propagation in experimental and natural scrapie in sheep with different PrP genotypes. Vet Pathol 42: 258-274.
  • Foster JD, Hope J, Fraser H (1993) Transmission of bovine spongiform encephalopathy to sheep and goats. Vet Rec 133: 339-341.
  • Foster JD, Parnham DW, Hunter N, Bruce M (2001) Distribution of the prion protein in sheep terminally affected with BSE following experimental oral transmission. J Gen Virol 82: 2319-2326.
  • Gonzalez L, Martin S, BegaraMcGorum I, Hunter N, Houston F, Simmons M, Jeffrey M (2002) Effects of agent strain and host genotype on PrP accumulation in the brain of sheep naturally and experimentally affected with scrapie. J Comp Pathol 126: 17-29.
  • Grassi J, Comoy E, Simon S, Créminon C, Frobert Y, Trapman S, Schimmel H, Hawkins SAC, Moynagh J, Deslys JP, Wells GAH (2001) Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue. Vet Rec 149: 577-582.
  • Groschup MH, Beekes M, McBride PA, Hardt M, Hainfellner JA, Budka H (1999) Deposition of disease-associated prion protein involves the peripheral nervous system in experimental scrapie. Acta Neuropathol 98: 453-457.
  • Hamir A, Miller J, Cutlip R (2004) Failure to detect prion protein (PrPres) by immunohistochemistry in striated muscle tissues of animals experimentally inoculated with agents of transmissible spongiform encephalopathy. Vet Pathol 41: 78-81.
  • Houston EF, Gravenor MB (2003) Clinical signs in sheep experimentally infected with Scrapie and BSE. Vet. Rec 152: 333-334.
  • Houston F, Goldmann W, Chong A, Jeffrey M, Gonzàlez L, Foster J, Parnham D, Hunter N (2003) BSE in sheep bred for resistance to infection. Nature 423: 98.
  • Hunter N (1997) PrP genetics in sheep and the application for scrapie and BSE. Trends Microbiol 5: 331-334.
  • Hunter N (2003) Scrapie and experimental BSE in sheep. Brit Med Bull 66: 171-183.
  • Ikeda T, Horiuchi M, Ishiguro N, Muramatsu Y, Uwe GG, Shinagawa M (1995) Amino acid polymorphisms of PrP with references to onset of scrapie in Suffolk and Corriedale sheep in Japan. J Gen Virol 76: 2577-2581.
  • Jeffrey M, McGovern G, Martin S, Goodsir CM, Brown KL (2000) Cellular and sub-cellular localisation of PrP in the lymphoreticular system of mice and sheep. Arch Virol (suppl): 23-38.
  • Jeffrey M, Ryder S, Martin S, Hawkins SAC, Terry L, BerthelinBaker C, Bellworthy SJ (2001) Oral inoculation of sheep with the agent of bovine spongiform encephalopathy (BSE). 1. Onset and distribution of disease-specific PrP accumulation in brain and viscera. J Comp Pathol 124: 280-289.
  • Lezmi S, Bencsik A, Baron T (2001) CNA42 monoclonal antibody identifies FDC as PrPsc accumulating cells in the spleen of scrapie affected sheep. Vet Immunol Immunopathol 82: 1-8.
  • Lezmi S, Bencsik A, Monks E, Petit T, Baron T (2003) First case of feline spongiform encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland. Histochem Cell Biol 119: 415-422.
  • Lezmi S, Martin S, Simon S, Comoy E, Bencsik A, Deslys JP, Grassi J, Jeffrey M, Baron T (2004) Comparative molecular analysis of the abnormal prion protein in field scrapie cases and experimental bovine spongiform encephalopathy in sheep by use of Western blotting and immunohistochemical methods. J Virol 78: 3654-3662.
  • Madec J, Simon S, Lezmi S, Bencsik A, Grassi J, Baron T (2004) Abnormal prion protein in genetically resistant sheep from a scrapie-infected flock. J Gen Virol 85: (Pt 11) 3483-3486.
  • Philippe S, Ducrot C, Roy P, Remontet L, Jarrige N, Calavas D (2005) Sheep feed and scrapie, France. Emerg Infect Dis 11: 1274-129.
  • Ryder SJ, Spencer YI, Bellerby PJ, March SA (2001) Immunohistochemical detection of PrP in the medulla oblongata of sheep: the spectrum of staining in normal and scrapie-affected sheep. Vet Rec 148: 7-13.
  • Thomzig A, Cardone F, Kruger D, Pocchiari M, Brown P, Beekes M (2006) Pathological prion protein in muscles of hamsters and mice infected with rodent-adapted BSE or vCJD. J Gen Virol 87: 251-254.
  • van Keulen LJM, Schreuder BEC, Vromans MEW, Langeveld JPM, Smits MA (1999) Scrapie-associated prion protein in the gastro-intestinal tract of sheep with natural scrapie. J Comp Pathol 121: 55-63.
  • Wilesmith JW, Ryan JBM, Hueston WD (1992) Bovine spongiform encephalopathy: case-control studies of calf feeding practices and meat and bonemeal inclusion in proprietary concentrates. Res Vet Sci 52: 325-331.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p399kz
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