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2006 | 53 | 2 | 303-309
Article title

An approach for the identification of microRNA with an application to Anopheles gambiae

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EN
Abstracts
EN
MicroRNAs (miRNAs) are an abundant class of 20-27 nt long noncoding RNAs, involved in post-transcriptional regulation of genes in eukaryotes. These miRNAs are usually highly conserved between the genomes of related organisms and their pre-miRNA transcript, about 60-120 nt long, forms extended stem-loop structure. Keeping these facts in mind miRsearch is developed which relies on searching the homologues of all known miRNAs of one organism in the genome of a related organism allowing few mismatches depending on the phylogenetic distance between them, followed by assessing for the capability of formation of stem-loop structure. The precursor sequences so obtained were then screened through the RNA folding program MFOLD selecting the cut-off values on the basis of known Drosophila melanogaster pre-miRNAs. With this approach, about 91 probable candidate miRNAs along with pre-miRNAs were identified in Anopheles gambiae using known D. melanogaster miRNAs. Out of these, 41 probable miRNAs have 100% similarity with already known D. melanogaster miRNAs and others were found to be at least 85% similar to the miRNAs of various other organisms.
Keywords
Publisher

Year
Volume
53
Issue
2
Pages
303-309
Physical description
Dates
published
2006
received
2006-02-17
revised
2006-04-23
accepted
2006-05-08
(unknown)
2006-06-12
Contributors
  • Human Genetics & Genomics Group, Indian Institute of Chemical Biology, Jadavpur, Kolkata, India
  • Human Genetics & Genomics Group, Indian Institute of Chemical Biology, Jadavpur, Kolkata, India
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p303kz
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