Glutathione and GSH-dependent enzymes in patients with liver cirrhosis and hepatocellular carcinoma

• Authors: Hanna Czeczot , Dorota Ścibior , Michał Skrzycki , Małgorzata Podsiad
• Languages of publication: EN

Abstracts

EN

We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.

Keywords

EN

malondialdehyde; glutathione S-transferase; glutathione reductase; liver cirrhosis; glutathione peroxidase; glutathione; hepatocellular carcinoma

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 1
• Pages: 237-242

Dates

published

2006

received

2005-06-23

revised

2005-10-18

accepted

2005-12-16

(unknown)

2006-01-09

Contributors

author

Hanna Czeczot

• Chair and Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

author

Dorota Ścibior

• Chair and Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

author

Michał Skrzycki

• Chair and Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

author

Małgorzata Podsiad

• Chair and Department of Biochemistry, Medical University of Warsaw, Warszawa, Poland

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