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2006 | 53 | 1 | 169-178
Article title

Mitochondria-associated satellite I RNA binds to hnRNP K protein

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EN
Abstracts
EN
hnRNP K protein, which localizes to the nucleus, cytoplasm and mitochondria, is involved in the various cellular processes that compose gene expression. We used a SAGE-based assay to profile RNAs associated with hnRNP K protein in rat mitochondria. RNA was isolated from mitoplasts obtained from highly purified and RNase-treated mitochondria. Total RNA and RNA associated with hnRNP K protein were then used as input material for generating two SAGE libraries. Mitochondrion-derived tags isolated from the total mitoplast RNA library represented 86.3%, while those isolated from the library constructed from RNA associated with hnRNP K protein represented only 28.2% of selected tags. Thus, an unexpected number of nuclear-encoded RNAs were purified from mitochondria. Many of these transcripts were co-purified with hnRNP K protein, and high levels of nuclear-encoded RNAs co-immunoprecipitating with K protein corresponded to elevated hnRNP K protein levels of the organelle. The most abundant RNAs that were co-purified with hnRNP K protein represented transcripts originating from satellite I DNA. While satellite I RNA levels were higher in the nucleus and cytoplasm than in mitochondria, the most abundant binding of satellite I transcripts to hnRNP K protein was found in mitochondria. The role of satellite I RNA in mitochondria remains to be elucidated.
Publisher

Year
Volume
53
Issue
1
Pages
169-178
Physical description
Dates
published
2006
received
2005-08-31
revised
2005-12-28
accepted
2006-01-02
(unknown)
2006-02-23
Contributors
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
author
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
author
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
  • Department of Gastroenterology, Medical Center for Postgraduate Education and the Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p169kz
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