Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher

• Authors: Yasuhiro Kambayashi , Susumu Takekoshi , Minoru Nakano , Masafumi Shibamori , Yoshiaki Hitomi , Keiki Ogino
• Languages of publication: EN

Abstracts

EN

We recently reported that kinobeon A, produced from safflower cells, suppressed the free radical-induced damage of cell and microsomal membranes. In the present study, we investigated whether kinobeon A quenches singlet oxygen, another important active oxygen species. Kinobeon A inhibited the singlet oxygen-induced oxidation of squalene. The second-order rate constant between singlet oxygen and kinobeon A was 1.15 × 1010 M-1s-1 in methanol containing 10% dimethyl sulfoxide at 37°C. Those of α-tocopherol and β-carotene, which are known potent singlet oxygen quenchers, were 4.45 × 108 M-1s-1 and 1.26 × 1010 M-1s-1, respectively. When kinobeon A was incubated with a thermolytic singlet oxygen generator, its concentration decreased. However, this change was extremely small compared to the amount of singlet oxygen formed and the inhibitory effect of kinobeon A on squalene oxidation by singlet oxygen. In conclusion, kinobeon A was a strong singlet oxygen quencher. It reacted chemically with singlet oxygen, but it was physical quenching that was mainly responsible for the elimination of singlet oxygen by kinobeon A. Kinobeon A is expected to have a preventive effect on singlet oxygen-related diseases of the skin or eyes.

Keywords

EN

kinobeon A; quencher; singlet oxygen; safflower; endoperoxide; antioxidant

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 4
• Pages: 903-908

Dates

published

2005

received

2005-08-10

revised

2005-09-8

accepted

2005-09-9

(unknown)

2005-10-25

Contributors

author

Yasuhiro Kambayashi

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan

author

Susumu Takekoshi

• Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan

author

Minoru Nakano

• Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories, Tekasaki, Japan

author

Masafumi Shibamori

• Third Institute of New Drug Discovery, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan

author

Yoshiaki Hitomi

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan

author

Keiki Ogino

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan

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