The role of benzoate secreted by Desulfotomaculum acetoxidans DSM 771 in sulfate uptake

• Authors: Lucyna Pawłowska-Ćwięk , Ryszard Pado
• Languages of publication: EN

Abstracts

EN

This work was designed to find the cause of the delay in hydrogen sulfide dissimilation in Desulfotomaculum acetoxidans DSM 771, which is dependent on the sulfate uptake. This bacterium grown without addition of any aromatic compound was shown by spectrum analysis with the methylene method to contain hydroxy-benzoate derivatives. The presence of these compounds was confirmed by HPLC in fractions obtained from cell walls after 15 days of culture. The test with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt seemed to indicate the presence of peroxidase, which probably oxidized benzoate to its hydroxy derivatives. The test with 5-sulfo-salicylic acid proved the ability of the investigated strain to utilize arylsulfates and to reduce sulfate group to hydrogen sulfide. On the basis of the above data, we propose the following sequence of reactions: 1, benzoate secretion; 2, benzoate hydroxylation; 3, sulfonation of hydroxy-benzoate derivatives.

Keywords

EN

sulfonation; benzoate secretion; oxidation

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 4
• Pages: 797-802

Dates

published

2005

received

2004-01-30

revised

2005-03-24

accepted

2005-10-13

(unknown)

2005-10-25

Contributors

author

Lucyna Pawłowska-Ćwięk

• Pedagogical University of Cracow, Department of Microbiology, Kraków, Poland

author

Ryszard Pado

• Pedagogical University of Cracow, Department of Microbiology, Kraków, Poland

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