Definition of immunogenic carbohydrate epitopes.

• Authors: Katharina Paschinger , Gustáv Fabini , David Schuster , Dubravko Rendić , Iain B H Wilson
• Languages of publication: EN

Abstracts

EN

Carbohydrates are known as sources of immunological cross-reactivity of allergenic significance. In celery and in cypress pollen, the major allergens Api g 5 and Cup a 1 are recognised by antisera raised against anti-horseradish peroxidase and by patients' IgE which apparently bind carbohydrate epitopes; mass spectrometric analysis of the tryptic peptides and of their N-glycans showed the presence of oligosaccharides carrying both xylose and core α1,3-fucose residues. Core α1,3-fucose residues are also a feature of invertebrates: genetic and biochemical studies on the fruitfly Drosophila melanogaster, the parasitic trematode Schistosoma mansoni and the nematode worm Caenorhabditis elegans indicate that these organisms possess core α1,3-fucosyltransferases. Various experiments have shown that fucosyltransferases from both fly and worm are responsible in vivo and in vitro for the synthesis of N-glycans which cross-react with anti-horseradish peroxidase; thus, we can consider these enzymes as useful tools in generating standard compounds for testing cross-reactive carbohydrate epitopes of allergenic interest.

Keywords

EN

cross-reactivity; allergy; Caenorhabditis; plant; Drosophila

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 3
• Pages: 629-632

Dates

published

2005

received

2005-03-15

accepted

2005-08-03

revised

2005-08-03

Contributors

author

Katharina Paschinger

• Department für Chemie, Universität für Bodenkultur, Wien, Austria

author

Gustáv Fabini

• Department für Chemie, Universität für Bodenkultur, Wien, Austria

author

David Schuster

• Department für Chemie, Universität für Bodenkultur, Wien, Austria

author

Dubravko Rendić

• Department für Chemie, Universität für Bodenkultur, Wien, Austria

author

Iain B H Wilson

• Department für Chemie, Universität für Bodenkultur, Wien, Austria

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