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2005 | 52 | 3 | 629-632
Article title

Definition of immunogenic carbohydrate epitopes.

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EN
Abstracts
EN
Carbohydrates are known as sources of immunological cross-reactivity of allergenic significance. In celery and in cypress pollen, the major allergens Api g 5 and Cup a 1 are recognised by antisera raised against anti-horseradish peroxidase and by patients' IgE which apparently bind carbohydrate epitopes; mass spectrometric analysis of the tryptic peptides and of their N-glycans showed the presence of oligosaccharides carrying both xylose and core α1,3-fucose residues. Core α1,3-fucose residues are also a feature of invertebrates: genetic and biochemical studies on the fruitfly Drosophila melanogaster, the parasitic trematode Schistosoma mansoni and the nematode worm Caenorhabditis elegans indicate that these organisms possess core α1,3-fucosyltransferases. Various experiments have shown that fucosyltransferases from both fly and worm are responsible in vivo and in vitro for the synthesis of N-glycans which cross-react with anti-horseradish peroxidase; thus, we can consider these enzymes as useful tools in generating standard compounds for testing cross-reactive carbohydrate epitopes of allergenic interest.
Publisher

Year
Volume
52
Issue
3
Pages
629-632
Physical description
Dates
published
2005
received
2005-03-15
accepted
2005-08-03
revised
2005-08-03
Contributors
  • Department für Chemie, Universität für Bodenkultur, Wien, Austria
  • Department für Chemie, Universität für Bodenkultur, Wien, Austria
  • Department für Chemie, Universität für Bodenkultur, Wien, Austria
  • Department für Chemie, Universität für Bodenkultur, Wien, Austria
  • Department für Chemie, Universität für Bodenkultur, Wien, Austria
References
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv52i3p629kz
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