Effect of deletion mutation on the recombination activity of Cre recombinase

• Authors: Liu Rongrong , Wang Lixia , Lin Zhongping
• Languages of publication: EN

Abstracts

EN

Cre recombinase from bacteriophage P1 is widely used in both in vitro and in vivo DNA manipulations. Based on a structural and functional analysis, three deleted cre mutants were constructed and expressed in Escherichia coli. Mutated recombinases were purified and their recombination activities were determined in vitro. Our results revealed that the mutant with amino-terminal deletion retains the recombination activity as high as wild type Cre; however, the carboxy-terminal deletion and the middle region deletion both lead to a complete loss of the recombinase function.

Keywords

EN

Cre recombinase; deletion mutation; in vitro recombination assay

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 2
• Pages: 541-544

Dates

published

2005

received

2004-12-14

revised

2005-01-31

accepted

2005-02-02

(unknown)

2005-05-15

Contributors

author

Liu Rongrong

• The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R.China

author

Wang Lixia

• The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R.China

author

Lin Zhongping

• The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R.China

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