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2005 | 52 | 2 | 541-544
Article title

Effect of deletion mutation on the recombination activity of Cre recombinase

Content
Title variants
Languages of publication
EN
Abstracts
EN
Cre recombinase from bacteriophage P1 is widely used in both in vitro and in vivo DNA manipulations. Based on a structural and functional analysis, three deleted cre mutants were constructed and expressed in Escherichia coli. Mutated recombinases were purified and their recombination activities were determined in vitro. Our results revealed that the mutant with amino-terminal deletion retains the recombination activity as high as wild type Cre; however, the carboxy-terminal deletion and the middle region deletion both lead to a complete loss of the recombinase function.
Publisher

Year
Volume
52
Issue
2
Pages
541-544
Physical description
Dates
published
2005
received
2004-12-14
revised
2005-01-31
accepted
2005-02-02
(unknown)
2005-05-15
Contributors
author
  • The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R.China
author
  • The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R.China
author
  • The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, P.R.China
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv52i2p541kz
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