PL EN


Preferences help
enabled [disable] Abstract
Number of results
2005 | 52 | 2 | 515-525
Article title

Small high density lipoprotein subclasses: some of their physico-chemical properties and stability in solution.

Content
Title variants
Languages of publication
EN
Abstracts
EN
Small high density lipoproteins (SHDL) contribute to the protection from atherosclerosis, but detailed information about their properties is not available yet. We isolated four of the smallest HDL subclasses that contain apoA-I alone, the small lipoprotein A-I (SLpAI), by their separation on gradient polyacrylamide gel followed by electroelution. Their physico-chemical properties were calculated from their displacement in non-denaturing gradient polyacrylamide gel under the effect of electrical potential. The properties are: Stokes' radii 2.96-3.56 nm; molecular masses 42-70 kDa; net negative charge 7.2-13.5; surface charge densities 3139-4069 -esu∙˙cm^(-2); surface potentials 10.6-15.7 -mV; coefficients of friction 5.74-6.90 × 10-8 g∙˙s^(-1); and diffusion coefficients 5.76-6.94 7× 10-7 cm^2∙˙s^(-1). We found that these particles were of low stability as they underwent molecular transformation into larger particles on storage. The estimated dimensions of these particles do not support ellipsoidal shape, therefore, the most probable shape is spherical; consequently, their hydrated characteristics were estimated. We conclude that these particles have high values of negative surface charge and diffusion coefficients, and are of low stability. Their small Stokes' radii were similar to each other and they are spherical and highly hydrated.
Year
Volume
52
Issue
2
Pages
515-525
Physical description
Dates
published
2005
received
2004-04-30
revised
2004-10-19
accepted
2004-12-15
(unknown)
2005-05-31
References
  • Armen R, Uitto OD, Feller SE (1998) Phospholipid component volumes: determination and application to bilayer structure calculations. Biophys J 75: 734-744.
  • Atmeh RF (1989) Concentration of dilute solutions of macromolecules by electrophoresis. Anal Biochem 182: 315-318.
  • Atmeh RF (1990) Isolation and identification of HDL particles of low molecular weight. J Lipid Res 31: 1771-1780.
  • Atmeh RF, Abuharfeil N (1993) Electroblotting of proteins on agarose gel containing specific antibodies. Electrophoresis 14: 69-72.
  • Atmeh RF, Robenek H (1996) Measurement of small high density lipoprotein subclass by an improved immunoblotting technique. J Lipid Res 37: 2461-2469.
  • Atmeh RF, Abuharfeil NM, Al-Sukhon SN, Abo-Shehada MN (1991) Electrophoretic elution of macromolecules from polyacrylamide or agarose gels. Prep Biochem 21: 1-9.
  • Barter PJ, Connor WE (1976) The transport of esterified cholesterol in plasma high density lipoproteins of human subjects: a mathematical model. J Lab Clin Med 88: 627-639.
  • Blanche PJ, Gong EL, Forte TM, Nichols AV (1981) Characterization of human high density lipoproteins by gradient gel electrophoresis. Biochim Biophys Acta 665: 408-419.
  • Braschi S, Neville TA-M, Vohl M-C, Sparks DL (1999) Apolipoprotein A-I charge and conformation regulate the clearance of reconstituted high density lipoprotein in vivo. J Lipid Res 40: 522-532.
  • Brouillette CB, Anantharamaiah GM, Engler JA, Borhani DW (2001) Structural models of human apolipoprotein A-I: a critical analysis and review. Biochim Biophys Acta 1531: 4-46.
  • Castro GR, Fielding CJ (1988) Early incorporation of cell-derived cholesterol into pre-β-migrating high density lipoprotein. Biochemistry 27: 25-29.
  • Chen C, Applegate K, King WC, Glomset JA, Norum KR, Gjone E (1984) A study of the small spherical high density lipoproteins of patients afflicted with familial lecithin:cholesterol acyltransferase deficiency. J Lipid Res 25: 269-282.
  • Cheung MC, Albers JJ (1984) Characterization of lipoprotein particles isolated by immuno-affinity chromatography, particles containing A-I and A-II and particles containing A-I but no A-II. J Biol Chem 259: 12201-12209.
  • Chiesa G, Parolini C, Canvesi M, Colombo N, Sirtori CR, Fumagalli R, Franceschini G, Bernini F (1998) Human apolipoproteins A-I and A-II in cell cholesterol efflux: studies with transgenic mice. Arterioscler Thromb Vasc Biol 18: 1417-1423.
  • Chiu SW, Jakobsson E, Subramaniam S, Scott L (1999) Combined Monte Carlo and molecular dynamics simulation of fully hydrated dioleyl and palmitoyl-oleyl phosphatidylcholine lipid bilayers. Biophys J 77: 2462-2469.
  • Collet X, Perret B, Simard G, Raffai E, Marcel YL (1991).Differential effects of lecithin and cholesterol on the immunoreactivity and conformation of apolipoprotein A-I in high density lipoproteins. J Biol Chem 266: 9145-9152.
  • Colvin PL, Parks JS (1999) Metabolism of high density lipoprotein subfractions. Curr Opin Lipidol 10: 309-314.
  • Davidson WS, Rodriguez WV, Lund-Katz S, Johnson WJ (1995) Effects of acceptor particle size on the efflux of cellular free cholesterol. J Biol Chem 270: 17106-17113.
  • Demeler B, Saber H (1998) Determination of molecular parameters by fitting sedimentation data to finite-element solutions of Lamm equation. Biophys J 74: 444-454.
  • Dergunov AD, Dobrestov GE, Visvikis S, Siest G (2001) Protein-lipid interactions in reconstituted high density lipoproteins: apolipoprotein and cholesterol influence. Chem Phys Lipids 113: 67-82.
  • Desrumaux C, Athias A, Masson D, Gambert P, Lallemant C, Lagrost L (1998) Influence of the electrostatic charge of lipoprotein particles on the activity of human plasma phospholipid transfer protein. J Lipid Res 39: 131-142.
  • Dory L, Sloop CH, Roheim PS (1986) Interstitial fluid (peripheral lymph) lipoproteins. Methods Enzymol 129: 660-678.
  • Duverger N, Rader D, Duchateau P, Fruchart JC, Castro G, Brewer HB (1993) Biochemical characterization of the three major subclasses of lipoprotein A-I preparatively isolated from human plasma. Biochemistry 32: 12372-12379.
  • Fielding PE, Fielding CJ (1980) A cholesteryl ester transfer complex in human plasma. Proc Natl Acad Sci USA 77: 3327-3330.
  • Fielding CJ, Fielding PE (1981) Evidence for a lipoprotein carrier in human plasma catalyzing sterol efflux from cultured fibroblasts and its relationship to lecithin:cholesterol acyltransferase. Proc Natl Acad Sci USA 78: 3911-3914.
  • Fielding PE, Kawano M, Catapano AL, Zoppo A, Marcovina S, Fielding CJ (1994) Unique epitope of apolipoprotein A-I expressed in pre-beta-1 high density lipoprotein and its role in the catalyzed efflux of cellular cholesterol. Biochemistry 33: 6981-6985.
  • Giddings JC (1991) Unified Separation Science, pp 155-156. Wiley, New York.
  • Gillotte KL, Davidson WS, Lund-Katz S, Rothblat GH, Phillips MC (1998) Removal of cellular cholesterol by pre-β-HDL involves plasma membrane microsolubilization. J Lipid Res 39: 1918-1928.
  • Glomset JA (1968) The plasma lecithin:cholesterol acyltransferase reaction. J Lipid Res 9: 155-167.
  • Handwerger S, Datta G, Richardson B, Schmidt CM, Siddiqi T, Turzai L, Anantharamaiah GM (1999) Pre-β-HDL stimulates placental lactogen release from human trophoblast cells. Am J Physiol 276 Pt1: E384-389.
  • Havel RJ, Eder HA, Bragdon JH (1955) The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 34: 1345-1353.
  • Haynes MP, Phillips MC, Rothblat GH (2000) Efflux of cholesterol from different cellular pools. Biochemistry 39: 4508-4517.
  • Ishida BY, Frolich J, Fielding CJ (1987) Prebeta-migrating high density lipoprotein: quantitation in normal and hyperlipidemic plasma by solid phase radioimmunoassay following electrophoretic transfer. J Lipid Res 28: 778-786.
  • Jaspard B, Collet X, Barbaras R, Manent J, Vieu C, Parinaud J, Chap H, Perre B (1996) Biochemical characterization of pre-β1-HDL from human ovarian follicular fluid: evidence for the presence of a lipid core. Biochemistry 35: 1352-1357.
  • Jonas A, Wald JH, Harms Toohill KL, Krul E, Kezzdy KE (1990) Apolipoprotein A-I structure and lipid properties in homogeneous, reconstituted spherical and discoidal high density lipoprotein. J Biol Chem 265: 22123-22129.
  • Koln AE, Segrest JP, Harvey SC (2002) Molecular dynamics simulation on discoidal HDL particles suggest a mechanism for rotation in the apoA-I belt model. J Mol Biol 324: 703-721.
  • Lamm O (1929) Die Differentialgleichung der Ultracentrifugierung. Ark Mat Astron Fys 21B: 1-4.
  • Li Z, McNamara JR, Ordovas JM, Schaefer EJ (1994) Analysis of high density lipoproteins by a modified gradient gel electrophoresis method. J Lipid Res 35: 1698-1711.
  • Lusa S, Jauhainen M, Mesto J, Somerharju P, Ehnholm C (1996) The mechanism of human plasma phospholipid transfer protein-induced enlargement of high density lipoprotein particles: evidence for particle fusion. Biochem J 313: 275-282.
  • Marcovina S, Fantappie S, Zoppo A, Franceschini G, Catapano AL (1990) Immunochemical characterization of six monoclonal antibodies to human apolipoprotein A-I: epitope mapping and expression. J Lipid Res 31: 375-384.
  • Miller NE (1990) High density lipoprotein metabolism and its role in lipid transport. Eur Heart J 11 Suppl H: 1-3.
  • Murzyn K, Rog T, Jezierski G, Takaoka Y, Pasenkiewicz-Gierula M (2001) Effects of phospholipid unsaturation on the membrane/water interface: a molecular simulation study. Biophys J 81: 170-183.
  • Nanjee MN, Brinton EA (2000) Very small apolipoprotein A-I-containing particles from human plasma: isolation and quantification by high-performance size-exclusion chromatography. Clin Chem 46: 207-223.
  • Ohta T, Saku K, Takata K, Nakamura R, Ikeda Y, Matsuda I (1995) Different effects of subclasses of HDL containing apoA-I but not apoA-II (LpA-I) on cholesterol esterification in plasma and net cholesterol efflux from foam cells. Arterioscler Thromb Vasc Biol 15: 956-962.
  • Oram JF, Albers JJ, Cheung MC, Bierman EL (1981) The effects of subfractions of high density lipoprotein on cholesterol efflux from cultured fibroblasts. Regulation of low density lipoprotein receptor activity. J Biol Chem 256: 8348-8356.
  • Patsch W, Schonfeld G, Gotto AM, Patsch JR (1980) Characterization of human high density lipoproteins by zonal ultracentrifugation. J Biol Chem 255: 3178-3185.
  • Petit E, Ayrault-Jarrier M, Pastier D, Robin H, Polonovski J, Aragon I, Hervaud E, Pau B (1987) Monoclonal antibodies to human apolipoprotein A-I: characterization and application as structural probes for apolipoprotein A-I and high density lipoprotein. Biochim Biophys Acta 919: 287-296.
  • Pio F, De Loof H, Vu Dac N, Clavy V, Fruchart JC, Rosseneu M (1988) Immunochemical characterization of two antigenic sites on human apolipoprotein A-I: localization and lipid modulation of these epitopes. Biochim Biophys Acta 959: 160-168.
  • Phillips JC, Wriggers W, Li Z, Jonas A, Schulten K (1997) Prediction of the structure of apolipoprotein A-I in reconstituted high-density lipoprotein disks. Biophys J 73: 2337-2346.
  • Rog T, Murzyn K, Pasenkiewicz-Gierula M (2003) Molecular dynamics simulations of charged and neutral lipid bilayers: treatment of electrostatic interactions. Acta Biochim Polon 50: 789-798.
  • Rothblat GH, de la Llera-Moya M, Atger V, Kellner-Weibel G, Williams DL, Phillips MC (1999) Cell cholesterol efflux: integration of old and new observations provides new insights. J Lipid Res 40: 781-796.
  • Rothe GM (1991) Determination of the size, isomeric nature and net charge of enzymes by pore gradient gel electrophoresis. In Advances in Electrophoresis. Chrambach A, Dunn MJ, Radola BJ, eds, vol 4, pp 251-358. VCH, Weinheim.
  • Rothe GM (1994) Electrophoresis of Enzymes, Laboratory Methods, pp 104-119. Springer Verlag, Berlin.
  • Saku K, von Eckardstein A, Zhang B, Liu R, Jimi S, Ou J, Ohta T, Assmann G, Arakawa A (1999) In vivo kinetics of human apolipoprotein A-I variants in rabbits. Eur J Clin Invest 29: 196-203.
  • Schwartz FT, Paltauf F (1976) Studies on the interaction of cholesterol with diester- and dietherlecithin. Chem Phys Lipids 17: 423-434.
  • Sparks DL, Phillips MC (1992) Quantitative measurement of lipoprotein surface charge by agarose gel electrophoresis. J Lipid Res 33: 123-130.
  • Sparks DL, Phillips MC, Lund-Katz S (1992a) The conformation of apolipoprotein A-I in discoidal and spherical recombinant high density lipoprotein particles. 13C NMR studies of lysine ionization behavior. J Biol Chem 267: 25830-25838.
  • Sparks DL, Lund-Katz S, Phillips MC (1992b) The charge and structural stability of apolipoprotein A-I in discoidal and spherical recombinant high density lipoprotein particles. J Biol Chem 267: 25839-25847.
  • Sparks DL, Anantharamaiah GM, Segrest JP, Phillips MC (1995) Effect of the cholesterol content of reconstituted LpA-I on lecithin:cholesterol acyltransferase activity. J Biol Chem 270: 5151-5157.
  • Sparks DL, Frank PG, Braschi S, Noville TA, Marcel YL (1999) Effect of apolipoprotein A-I lipidation on the formation and function of pre-beta and alpha-migrating LpA-I particles. Biochemistry 38: 1727-1735.
  • Sviridov D, Miyazaki O, Theodore K, Hoang A, Fukamachi I, Nestel P (2002) Delineation of the role of pre-β1-HDL in cholesterol efflux using isolated pre-β1-HDL. Arterioscler Thromb Vasc Biol 22: 1482-1488.
  • Tricerri A, Corsico B, Toledo JD, Garda HA, Brenner RR (1998) Conformation of apolipoprotein A-I in reconstituted lipoprotein particles and particle-membrane interaction: effect of cholesterol. Biochim Biophys Acta 1391: 67-78.
  • Tristram-Nagle S, Petrache HI, Nagle J (1998) Structure and interactions of fully hydrated dioleoylphosphatidylcholine bilayers. Biophys J 75: 917-925.
  • Tu H, Klein ML, Tobias DJ (1998) Constant-pressure molecular dynamics investigation of cholesterol effects in a dipalmitoylphosphatidylcholine bilayer. Biophys J 75: 2147-2156.
  • Vitello LB, Scanu AM (1976) Studies on human serum high density lipoproteins. Self association of apolipoprotein A-I in aqueous solution. J Biol Chem 251: 1131-1136.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv52i2p515kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.