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2005 | 52 | 2 | 373-380
Article title

Structure, function, and regulation of myosin 1C.

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Myosin 1C, the first mammalian single-headed myosin to be purified, cloned, and sequenced, has been implicated in the translocation of plasma membrane channels and transporters. Like other forms of myosin I (of which eight exist in humans) myosin 1C consists of motor, neck, and tail domains. The neck domain binds calmodulins more tightly in the absence than in the presence of Ca^(2+). Release of calmodulins exposes binding sites for anionic lipids, particularly phosphoinositides. The tail domain, which has an isoelectic point of 10.5, interacts with anionic lipid headgroups. When both neck and tail lipid binding sites are engaged, the myosin associates essentially irreversibly with membranes. Despite this tight membrane binding, it is widely believed that myosin 1C docking proteins are necessary for targeting the enzyme to specific subcellular location. The search for these putative myosin 1C receptors is an active area of research.
Physical description
  • Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA;
  • Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA;
  • Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA;
  • Adams RJ, Pollard TD (1989) Binding of myosin I to membrane lipids. Nature 340: 565-568.
  • Allen LH, Aderem A (1995) A role for MARCKS, the alpha isozyme of protein kinase C and myosin I in zymosan phagocytosis by macrophages. J Exp Med 182: 829-840.
  • Anderson RG, Jacobson K (2002) A role for lipid shells in targeting proteins to caveolae, rafts, and other lipid domains. Science 296: 1821-1825.
  • Bahler M, Rhoads A (2002) Calmodulin signaling via the IQ motif. FEBS Lett 513: 107-113.
  • Barylko B, Wagner MC, Reizes O, Albanesi JP (1992) Purification and characterization of a mammalian myosin I. Proc Natl Acad Sci USA 89: 490-494.
  • Batters C, Arthur CP, Lin A, Porter J, Geeves MA, Milligan RA, Molloy JE, Coluccio LM (2004) Myo1c is designed for the adaptation response in the inner ear. EMBO J 23: 1433-14340.
  • Bose A, Robida S, Furcinitti PS, Chawla A, Fogarty K, Corvera S, Czech MP (2004) Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway. Mol Cell Biol 24: 5447-5458.
  • Breckler J, Burnside B (1994) Myosin I localizes to the midbody region during mammalian cytokinesis. Cell Motil Cytoskeleton 29: 312-320.
  • Buss F, Arden SD, Lindsay M, Luzio JP, Kendrick-Jones J (2001) Myosin VI isoform localized to clathrin-coated vesicles with a role in clathrin-mediated endocytosis. EMBO J 20: 3676-3684.
  • Buss F, Spudich G, Kendrick-Jones J (2004) Myosin VI: cellular functions and motor properties. Annu Rev Cell Dev Biol 20: 649-676.
  • Caroni P (2001) New EMBO members’ review: actin cytoskeleton regulation through modulation of PI(4,5)P(2) rafts. EMBO J 20: 4332-4336.
  • Chamberlain LH (2004) Detergents as tools for the purification and classification of lipid rafts. FEBS Lett 559: 1-5.
  • Claas C, Stipp CS, Hemler ME (2001) Evaluation of prototype transmembrane 4 superfamily protein complexes and their relation to lipid rafts. J Biol Chem 276: 7974-7984.
  • Coluccio LM, Geeves MA (1999) Transient kinetic analysis of the 130-kDa myosin I (MYR-1 gene product) from rat liver. A myosin I designed for maintenance of tension? J Biol Chem 274: 21575-21580.
  • Cox D, Berg JS, Cammer M, Chinegwundoh JO, Dale BM, Cheney RE, Greenberg S (2002) Myosin X is a downstream effector of PI(3)K during phagocytosis. Nat Cell Biol 4: 469-477.
  • De La Cruz EM, Ostap EM (2004) Relating biochemistry and function in the myosin superfamily. Curr Opin Cell Biol 16: 61-67.
  • Diefenbach TJ, Latham VM, Yimlamai D, Liu CA, Herman IM, Jay DG (2002) Myosin 1c and myosin IIB serve opposing roles in lamellipodial dynamics of the neuronal growth cone. J Cell Biol 158: 1207-1217.
  • Doberstein SK, Pollard TD (1992) Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba myosin IC. J Cell Biol 117: 1241-1249.
  • Ducluzeau PH, Fletcher LM, Vidal H, Laville M, Tavare JM (2002) Molecular mechanisms of insulin-stimulated glucose uptake in adipocytes. Diabetes Metab 28: 85-92.
  • El Mezgueldi M, Tang N, Rosenfeld SS, Ostap EM (2002) The kinetic mechanism of Myo1e (human myosin-IC). J Biol Chem 277: 21514-21521.
  • Fukuda M, Kuroda TS, Mikoshiba K (2002) Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J Biol Chem 277: 12432-12436.
  • Garcia JA, Yee AG, Gillespie PG, Corey DP (1998) Localization of myosin-Iβ near both ends of tip links in frog saccular hair cells. J Neurosci 18: 8637-8647.
  • Gillespie PG, Albanesi JP, Bahler M, Bement WM, Berg JS, Burgess DR, Burnside B, Cheney RE, Corey DP, Coudrier E, et al. (2001) Myosin-I nomenclature. J Cell Biol 155: 703-704.
  • Gillespie PG, Cyr JL (2004) Myosin-1c, the hair cell’s adaptation motor. Annu Rev Physiol 66: 521-545.
  • Golub T, Wacha S, Caroni P (2004) Spatial and temporal control of signaling through lipid rafts. Curr Opin Neurobiol 14: 542-550.
  • Graf B, Bahler M, Hilpela P, Bowe C, Adam T (2000) Functional role for the class IX myosin myr5 in epithelial cell infection by Shigella flexneri. Cell Microbiol 2: 601-616.
  • Hammer JA 3rd, Wu XS (2002) Rabs grab motors: defining the connections between Rab GTPases and motor proteins. Curr Opin Cell Biol 14: 69-75.
  • Hayden SM, Wolenski JS, Mooseker MS (1990) Binding of brush border myosin I to phospholipid vesicles. J Cell Biol 111: 443-451.
  • Hirono M, Denis CS, Richardson GP, Gillespie PG (2004) Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation. Neuron 44: 309-320.
  • Huber LA, Fialka I, Paiha K, Hunziker W, Sacks DB, Bahler M, Way M, Gagescu R, Gruenberg J (2000) Both calmodulin and the unconventional myosin Myr4 regulate membrane trafficking along the recycling pathway of MDCK cells. Traffic 1: 494-503.
  • Jay DG (1988) Selective destruction of protein function by chromophore-assisted laser inactivation. Proc Natl Acad Sci USA 85: 5454-5458.
  • Jay DG (2000) The clutch hypothesis revisited: ascribing the roles of actin-associated proteins in filopodial protrusion in the nerve growth cone. J Neurobiol 44: 114-125.
  • Jay DG, Keshishian H (1990) Laser inactivation of fasciclin I disrupts axon adhesion of grasshopper pioneer neurons. Nature 348: 548-550.
  • Jordan S, Rodgers W (2003) T cell glycolipid-enriched membrane domains are constitutively assembled as membrane patches that translocate to immune synapses. J Immunol 171: 78-87.
  • Korn ED (2000) Coevolution of head, neck, and tail domains of myosin heavy chains. Proc Natl Acad Sci USA 97: 12559-12564.
  • Mercer JA, Seperack PK, Strobel MC, Copeland NG, Jenkins NA (1991) Novel myosin heavy chain encoded by murine dilute coat colour locus. Nature 349: 709-713.
  • Nakanishi S, Yano H, Matsuda Y (1995) Novel functions of phosphatidylinositol 3-kinase in terminally differentiated cells. Cell Signal 7: 545-557.
  • Nebl T, Pestonjamasp KN, Leszyk JD, Crowley JL, Oh SW, Luna EJ (2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes. J Biol Chem 277: 43399-43409.
  • Pike LJ (2004) Lipid rafts: heterogeneity on the high seas. Biochem J 378: 281-292.
  • Pollard TD, Korn ED (1973) Acanthamoeba myosin. I. Isolation from Acanthamoeba castellanii of an enzyme similar to muscle myosin. J Biol Chem 248: 4682-4690.
  • Pralle A, Keller P, Florin EL, Simons K, Horber JK (2000) Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells. J Cell Biol 148: 997-1008.
  • Raposo G, Cordonnier MN, Tenza D, Menichi B, Durrbach A, Louvard D, Coudrier E (1999) Association of myosin Iα with endosomes and lysosomes in mammalian cells. Mol Biol Cell 10: 1477-1494.
  • Rayment I, Rypniewski WR, Schmidt-Base K, Smith R, Tomchick DR, Benning MM, Winkelmann DA, Wesenberg G, Holden HM (1993) Three-dimensional structure of myosin subfragment-1: a molecular motor. Science 261: 50-58.
  • Reizes O, Barylko B, Li C, Sudhof TC, Albanesi JP (1994) Domain structure of a mammalian myosin Iβ. Proc Natl Acad Sci USA 91: 6349-6353.
  • Saeki K, Miura Y, Aki D, Kurosaki T, Yoshimura A (2003) The B cell-specific major raft protein, Raftlin, is necessary for the integrity of lipid raft and BCR signal transduction. EMBO J 22: 3015-3026.
  • Seabra MC, Coudrier E (2004) Rab GTPases and myosin motors in organelle motility. Traffic 5: 393-399.
  • Sherr EH, Joyce MP, Greene LA (1993) Mammalian myosin Iα, Iβa, and Iγ: new widely expressed genes of the myosin I family. J Cell Biol 120: 1405-1416.
  • Shogomori H, Brown DA (2003) Use of detergents to study membrane rafts: the good, the bad, and the ugly. Biol Chem 384: 1259-1263.
  • Simons K, Vaz WL (2004) Model systems, lipid rafts, and cell membranes. Annu Rev Biophys Biomol Struct 33: 269-295.
  • Smart EJ, Ying YS, Mineo C, Anderson RG (1995) A detergent-free method for purifying caveolae membrane from tissue culture cells. Proc Natl Acad Sci USA 92: 10104-10108.
  • Song KS, Li S, Okamoto T, Quilliam LA, Sargiacomo M, Lisanti MP (1996) Co-purification and direct interaction of Ras with caveolin, an integral membrane protein of caveolae microdomains. Detergent-free purification of caveolae microdomains. J Biol Chem 271: 9690-9697.
  • Tang N, Lin T, Ostap EM (2002) Dynamics of myo1c (myosin-Iβ) lipid binding and dissociation. J Biol Chem 277: 42763-42768.
  • Tharaux PL, Bukoski RC, Rocha PN, Crowley SD, Ruiz P, Nataraj C, Howell DN, Kaibuchi K, Spurney RF, Coffman TM (2003) Rho kinase promotes alloimmune responses by regulating the proliferation and structure of T cells. J Immunol 171: 96-105.
  • Wagner MC, Barylko B, Albanesi JP (1992) Tissue distribution and subcellular localization of mammalian myosin I. J Cell Biol 119: 163-170.
  • Wang FS, Liu CW, Diefenbach TJ, Jay DG (2003) Modeling the role of myosin 1c in neuronal growth cone turning. Biophys J 85: 3319-28.
  • Wang FS, Wolenski JS, Cheney RE, Mooseker MS, Jay DG (1996) Function of myosin-V in filopodial extension of neuronal growth cones. Science 273: 660-663.
  • Warner CL, Stewart A, Luzio JP, Steel KP, Libby RT, Kendrick-Jones J, Buss F (2003) Loss of myosin VI reduces secretion and the size of the Golgi in fibroblasts from Snell’s waltzer mice. EMBO J 22: 569-579.
  • Watson RT, Kanzaki M, Pessin JE (2004) Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes. Endocr Rev 25: 177-204.
  • Williams R, Coluccio LM (1995) Phosphorylation of myosin-I from rat liver by protein kinase C reduces calmodulin binding. Biochem Biophys Res Commun 216: 90-102.
  • Wu H, Nash JE, Zamorano P, Garner CC (2002) Interaction of SAP97 with minus-end-directed actin motor myosin VI. Implications for AMPA receptor trafficking. J Biol Chem 277: 30928-30934.
  • Wu X, Bowers B, Wei, Kocher B, Hammer JA III (1997) Myosin V associates with melanosomes in mouse melanocytes: evidence that myosin V is an organelle motor. J Cell Sci 110: 847-859.
  • Yonezawa S, Yoshizaki N, Sano M, Hanai A, Masaki S, Takizawa T, Kageyama T, Moriyama A (2003) Possible involvement of myosin-X in intercellular adhesion: importance of serial pleckstrin homology regions for intracellular localization. Dev Growth Differ 45: 175-185.
  • Zhang H, Berg JS, Li Z, Wang Y, Lang P, Sousa AD, Bhaskar A, Cheney RE, Stromblad S (2004) Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat Cell Biol 6: 523-531.
  • Zhu T, Beckingham K, Ikebe M (1998) High affinity Ca2+ binding sites of calmodulin are critical for the regulation of myosin Iβ motor function. J Biol Chem 273: 20481-20486.
  • Zhu T, Sata M, Ikebe M (1996) Functional expression of mammalian myosin Iβ: analysis of its motor activity. Biochemistry 35: 513-522.
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