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2005 | 52 | 1 | 195-205
Article title

Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation.

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EN
Abstracts
EN
O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion.
Year
Volume
52
Issue
1
Pages
195-205
Physical description
Dates
published
2005
received
2004-09-14
accepted
2005-01-18
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv52i1p195kz
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