Preferences help
enabled [disable] Abstract
Number of results
2005 | 52 | 1 | 129-137
Article title

Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

Title variants
Languages of publication
Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptides resulting from protease digestion indicated that V2p is a derivative of Var1p. According to our hypothesis, V2p represents a readthrough product of the natural stop codon in VAR1 mRNA.
Physical description
  • Centre de Génétique Moléculaire, Laboratoire Propre 2167 du CNRS Associé à l'Université Pierre et Marie Curie, Paris, Cedex, France
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Altamura N, Groudinsky O, Dujardin G, Slonimski PP. (1992) NAM7 nuclear gene encodes a novel member of a family of helicases with a Zn-ligand motif and is involved in mitochondrial functions in Saccharomyces cerevisiae. J Mol Biol.; 224: 575-87.
  • Asher EB, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski PP. (1989) Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet.; 215: 517-28.
  • Boguta M, Mieszczak M, Zagorski W. (1988) Nuclear omnipotent suppressors of premature termination codons in mitochondrial genes affect the 37S mitoribosomal subunit. Curr Genet.; 13: 129-35.
  • Boguta M, Dmochowska A, Borsuk P, Wróbel K, Gargouri A, Lazowska J, Slonimski PP, Szczesniak B, Kruszewska A. (1992) NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal protein from chloroplasts bacteria and eukaryotes. Mol Cell Biol.; 12: 402-12.
  • Bonner WM, Laskey RA. (1974) A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem.; 46: 83-8.
  • Butow RA, Perlman PS, Grossman LI. (1985) The unusual var1 gene of yeast mitochondrial DNA. Science.; 228: 1496-501.
  • Butow RA, Zhu H, Perlman P, Conrad-Webb H. (1989) The role of a conserved dodecamer sequence in yeast mitochondrial gene expression. Genome.; 31: 757-60.
  • Claisse ML, Spyridakis A, Slonimski PP. (1977) Mutations of any one of three unlinked mitochondrial genetic loci box 1 box 4 and box 6 modify the structure of cytochrome b polypeptide(s). in: Mitochondria 1977. Brandlow W, Schweyen RJ, Wolf K, Kaudewitz F, eds, pp 337-44. Walter de Gruyter & Co. Berlin.
  • Claisse ML, Slonimski PP, Johnson J, Mahler HR. (1980) Mutations within an intron and its flanking sites: patterns of novel polypeptides generated by mutants in one segment of the cob-box region of yeast mitochondrial DNA. Mol Gen Genet.; 177: 375-87.
  • Cleveland DW, Fisher SG, Kirshner MW, Laemmli UK. (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem.; 252: 1102-6.
  • Davis SC, Ellis SR. (1995) Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into ribosomal subunits requires the mitochondrially encoded Var1 protein. Mol Gen Genet.; 247: 379-86.
  • Dujardin G, Lund P, Slonimski PP. (1984) The effect of paromomycin and [psi] on the suppression of mitochondrial mutations in Saccharomyces cerevisiae. Curr Genet.; 9: 21-30.
  • Ekwall K, Kermorgant M, Dujardin G, Groudinsky O, Slonimski PP. (1992) The NAM8 gene in Saccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed. Mol Gen Genet.; 233: 136-44.
  • Fiori A, Mason TL, Fox TD. (2003) Evidence that synthesis of the Saccharomyces cerevisiae mitochondrially encoded ribosomal protein Var1p may be membrane localized. Eukaryot Cell.; 2: 651-3.
  • Foury F, Roganti T, Lecremier N, Purnelle B. (1998) The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae. FEBS Lett.; 440: 325-31.
  • Fox TD, Weiss-Brummer B. (1980) Leaky+1 and -1 frameshift mutations at the same site in a yeast mitochondrial gene. Nature.; 288: 60-3.
  • Herbert CJ, Labouesse M, Dujardin G, Slonimski PP. (1988) The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases and are involved in mRNA splicing. EMBO J.; 7: 473-83.
  • Jacq C, Pajot P, Lazowska J, Dujardin G, Claisse M, Groudinsky O, Salle H, Grandchamp C, Labouesse M, Gargouri A, Guiard B, Spyridakis A, Dreyfus M, Slonimski PP. (1982) Role of introns in the yeast cytochrome b gene: cis and trans-acting signals intron manipulation expression and intergenic communications. in Mitochondrial Genes. Bandlow W, Kaudewitz F, eds, pp 155-83. Cold Spring Harbor University Press NY.
  • McKee EE, Poyton RO. (1984) Mitochondrial gene expression in Saccharomyces cerevisiae. I. Optimal conditions for protein synthesis in isolated mitochondria. J Biol Chem.; 259: 9320-31.
  • Kozlowski M, Zagorski W. (1988) Stable preparation of yeast mitochondria and mitoplasts synthesizing specific polypeptides. Anal Biochem.; 172: 382-91.
  • Kruszewska A, Slonimski PP. (1984a) Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria. I. Isolation localisation and allelism of suppressors. Curr Genet.; 9: 1-10.
  • Kruszewska A, Slonimski PP. (1984b) Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria. II. Specificity and extent of suppressor action. Curr Genet.; 9: 11-9.
  • Mieszczak M, Zagorski W. (1987) mim3 and nam3 omnipotent suppressor genes similarly affect the polypeptide composition of yeast mitoribosome. Biochimie.; 69: 531-7.
  • Namy O, Duchateau-Nguyen GM, Rousset JP. (2001) Translational readthrough of the PDE2 stop codon modulates cAMP levels in Saccharomyces cerevisiae. Mol Microbiol.; 43: 641-52.
  • Sanchirico ME, Fox TD, Mason TL. (1998) Accumulation of mitochondrially synthesized Saccharomyces cerevisiae Cox2p and Cox3p depends on targeting information in untranslated portions of their mRNAs. EMBO J.; 17: 5796-804.
  • Terpstra P, Zanders E, Butow R. (1979) The association of var1 with the 38 S mitochondrial ribosomal subunit in yeast. J Biol Chem.; 254: 12653-61.
  • Towpik J, Chacinska A, Ciesla M, Ginalski K, Boguta M. (2004) Mutations in the yeast MRF1 gene encoding mitochondrial release factor inhibit translation on mitochondrial ribosomes. J Biol Chem.; 279: 14096-103.
  • Wilhelm JM, Jessop JJ, Pettitt SE. (1978) Aminoglycoside anitibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cells. Biochemistry.; 17: 1149-53.
  • Zagorski W, Boguta M, Mieszczak M, Claisse ML, Guiard B, Spyridakis A, Slonimski PP. (1987a) Phenotypic suppression and nuclear accomodation of the mit- oxi1-V25 mutation in isolated yeast mitochondria. Curr Genet.; 12: 305-10.
  • Zagorski W, Kozlowski M, Mieszczak M, Spyridakis A, Claisse ML, Slonimski PP. (1987b) Protein synthesis in mitochondria from yeast strains carrying nam and mim suppressor genes. Biochimie.; 69: 517-29.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.