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2005 | 52 | 1 | 129-137
Article title

Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

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EN
Abstracts
EN
Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptides resulting from protease digestion indicated that V2p is a derivative of Var1p. According to our hypothesis, V2p represents a readthrough product of the natural stop codon in VAR1 mRNA.
Publisher

Year
Volume
52
Issue
1
Pages
129-137
Physical description
Dates
published
2005
received
2004-12-10
accepted
2005-02-03
Contributors
  • Centre de Génétique Moléculaire, Laboratoire Propre 2167 du CNRS Associé à l'Université Pierre et Marie Curie, Paris, Cedex, France
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv52i1p129kz
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