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2004 | 51 | 4 | 963-970
Article title

Analysis of ligand binding process using binding capacity concept.

Content
Title variants
Languages of publication
EN
Abstracts
EN
Binding capacity is the homotropic second derivative of the binding potential with respect to the chemical potential of the ligand. It provides a measure of steepness of the binding isotherm and represents the extent of cooperativity. In the present study, the shape of the binding capacity curve for various systems was investigated and the relation between binding capacity and the extent of cooperativity examined. In this regard, a novel linear graphical method was introduced for binding data analysis. The stoichiometry of binding and the extent of cooperativity can be determined by this method. This method has been successfully applied to various systems such as binding of oxygen to hemoglobin, warfarin to human serum albumin and dodecyltrimethylammonium bromide to α-amylase.
Publisher

Year
Volume
51
Issue
4
Pages
963-970
Physical description
Dates
published
2004
received
2003-10-10
revised
2004-06-28
accepted
2004-07-22
Contributors
author
  • Department of Chemistry, Isfahan University, Isfahan, Iran
author
  • Department of Chemistry, Islamic Azad University, Omidiye Unit, Omidiye, Iran
author
  • Department of Chemistry, Isfahan University, Isfahan, Iran
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i4p963kz
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