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2004 | 51 | 4 | 953-962
Article title

Benzodiazepine binding to mitochondrial membranes of the amoeba Acanthamoeba castellanii and the yeast Saccharomyces cerevisiae.

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EN
Abstracts
EN
Benzodiazepine binding sites were studied in mitochondria of unicellular eukaryotes, the amoeba Acathamoeba castellanii and the yeast Saccharomyces cerevisiae, and also in rat liver mitochondria as a control. For that purpose we applied Ro5-4864, a well-known ligand of the mitochondrial benzodiazepine receptor (MBR) present in mammalian mitochondria. The levels of specific [3H]Ro5-4864 binding, the dissociation constant (KD) and the number of [3H]Ro5-4864 binding sites (Bmax) determined for fractions of the studied mitochondria indicate the presence of specific [3H]Ro5-4864 binding sites in the outer membrane of yeast and amoeba mitochondria as well as in yeast mitoplasts. Thus, A. castellanii and S. cerevisiae mitochondria, like rat liver mitochondria, contain proteins able to bind specifically [3H]Ro5-4864. Labeling of amoeba, yeast and rat liver mitochondria with [3H]Ro5-4864 revealed proteins identified as the voltage dependent anion selective channel (VDAC) in the outer membrane and adenine nucleotide translocase (ANT) in the inner membrane. Therefore, the specific MBR ligand binding is not confined only to mammalian mitochondria and is more widespread within the eukaryotic world. However, it can not be excluded that MBR ligand binding sites are exploited efficiently only by higher multicellular eukaryotes. Nevertheless, the MBR ligand binding sites in mitochondria of lower eukaryotes can be applied as useful models in studies on mammalian MBR.
Year
Volume
51
Issue
4
Pages
953-962
Physical description
Dates
published
2004
received
2004-03-09
revised
2004-07-01
accepted
2004-10-28
References
  • Anholt RPH, De Souza EB, Oster-Granite ML, Snyder SH. (1985) Peripheral type of benzodiazepine receptors. Autoradiographic localization in whole body sections of neonatal rats. J Pharmacol Exp Ther.; 233: 517-28.
  • Antkiewicz-Michaluk L, Guidotti A, Krueger KE. (1988) Molecular characterization and mitochondrial density of a recognition site for peripheral-type of benzodiazepine ligands. Mol Pharmacol.; 34: 272-8.
  • Antos N, Budzińska M, Kmita H. (2001) An interplay between the TOM complex and porin isoforms in the yeast Saccharomyces cerevisiae mitochondria. FEBS Lett.; 500: 12-6.
  • Bar Ami S, Amiri Z, Fares F, Gawish M. (1994) Modulation of peripheral benzodiazepine receptors in female rat genital organs by various gonadal steroids. Life Sci.; 54: 1965-75.
  • Basile AS, Skolnick P. (1986) Subcellular localization of peripheral-type binding sites for benzodiazepines in rat brain. J Neurochem.; 46: 305-8.
  • Blachly-Dyson E, Song J, Wolfgang WJ, Colombini M, Forte M. (1997) Multicopy suppressors of phenotypes resulting from the absence of yeast VDAC encode a VDAC-like protein. Mol Cell Biol.; 17: 5227-38.
  • Black KL, Shiraishi T, Ikezaki K, Tabuchi K, Becker DP. (1994) Peripheral benzodiazepine stimulates secretion of growth hormone and mitochondrial proliferation in pituitary tumor GH cells. Neurol Res.; 16: 74-84.
  • Braestrup C, Squires RF. (1977) Specific benzodiazepine receptors in rat brain characterized by high affinity [3H]diazepam binding. Proc Natl Acad Sci USA.; 74: 3805-9
  • Brdiczka D, Pette D, Brunnrer G, Miller F. (1968) Compartmental dispersion of enzymes in rat liver mitochondria. Eur J Biochem.; 5: 294-304.
  • Dar DE, Weizman A, Karp L, Grinshpoon A, Bidder M, Kotler M, Tyano S, Bleich A, Gavish M. (1991) Platelet peripheral benzodiazepine receptors in repeated stress. Life Sci.; 48: 341-6.
  • Daum G, Bohni P, Schatz G. (1982) Import of proteins into mitochondria. J Biol Chem.; 257: 13028-33.
  • Garnier M, Dimchew AB, Boujard N, Price JM, Musto NA, Papadopoulos V. (1994) In vitro reconstitution of a functional peripheral type benzodiazepine receptor from mouse Leydig tumor cells. Mol Pharmacol.; 45: 201-11.
  • Gavish M, Bachman I, Shoukrun R, Katz Y, Veenman L, Weisinger G, Weizman A. (1999) Enigma of the peripheral benzodiazepine receptor. Pharmacol Rev.; 51: 629-50.
  • Gray MW, Burger G, Lang BE. (1999) Mitochondrial evolution. Science.; 283: 1476-81.
  • Hardwick M, Fertikh D, Culty M, Li H, Violic B, Papadopoulos V. (1999) Peripheral type benzodiazepine receptor (PBR) in human breast cancer. Cancer Res.; 59: 831-42.
  • Hirsch JD, Beyer CF, Malkowitz L, Beer B, Blume AJ. (1989) Mitochondrial benzodiazepine receptor mediates inhibition of mitochondrial respiratory control. Mol Pharmacol.; 35: 157-63.
  • Hirsch T, Decaudin D, Susin SA, Marchetti P, Larochette N, Rigon M, Kroemer G. (1998) PK 11195, a ligand of the mitochondrial benzodiazepine receptor facilitates the induction of apoptosis and reverses Bcl2 mediated cytoprotection. Exp Cell Res.; 241: 426-34
  • Hryniewiecka L. (1986) Malate oxidation in mitochondria of Acanthamoeba castellanii Neff. Bull Soc Sci Lett.; 25: 5-15.
  • Hryniewiecka L, Jenek J, Michejda J. (1978) Cyanide resistance in soil amoeba Acanthamoeba castellanii. In Plant Mitochondria. Ducet G, Lance C. eds, pp 307-314. Elsevier.
  • Hryniewiecka L, Domka-Popek A, Michejda J. (1979) Stimulation of respiration of Acanthamoeba castellanii by calcium ions. Bull Soc Sci Lett.; 19: 13-23.
  • Ikezaki K, Black KL. (1990) Stimulation of cell growth and DNA synthesis by peripheral benzodiazepine receptor. Cancer Lett.; 49: 115-20.
  • Kinnally KW, Zorov D, Antonenko N, Snyder SH, Mc Enery MW, Tedeschi H. (1993) Mitochondrial benzodiazepine receptor linked to inner membrane ion channels by nanomolar actions of ligands. Proc Natl Acad Sci USA.; 90: 1374-8.
  • Kmita H, Budzinska M. (2000) Involvement of the TOM complex in external NADH transport into yeast mitochondria depleted of mitochondrial porin 1. Biochim Biophys Acta.; 1509: 86-94.
  • Kroemer G, Zamzani N, Susin SA. (1997) Mitochondrial control of apoptosis. Immunol Today.; 18: 44-57.
  • Krueger KE. (1995) Molecular and functional properties of mitochondrial benzodiazepine receptors. Biochim Biophys Acta.; 1241: 453-70.
  • Lee AC, Xu X, Blachly-Dyson E, Forte M, Colombini M. (1998) The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane. J Membr Biol.; 161: 173-81.
  • Lenfant M, Haumont J, Zavala F. (1986) In vivo immunomodulating activity of PK 11195, a structurally ligand for peripheral benzodiazepine binding site. Int J Immunopharmacol.; 8: 825-9.
  • McEnery MW, Snowman AM, Trifiletti RR, Snyder SH. (1992) Isolation of the mitochondrial benzodiazepine receptor: association with the voltage-dependent anion channel and the adenine nucleotide carrier. Proc Natl Acad Sci USA.; 89: 3170-4.
  • Neff RJ. (1957) Purification, axenic cultivation and description of a soil amoeba Acanthamoeba sp. J Protozool.; 4: 176-82.
  • Neupert W, Paschen S. (2001) Protein import into mitochondria. IUBMB Life.; 52: 101-12.
  • Pellow S, File S. (1984) Behavioural actions of Ro5-4864: A peripheral-type benzodiazepine. Life Sci.; 35: 229-40
  • Python CP, Rossier MF, Vallotto MB, Capponi AM. (1993) Peripheral type benzodiazepines inhibit calcium channels and aldosterol production in adrenal glomerulosa cells. Endocrinology.; 132: 1489-96.
  • Riond J, Mattei MG, Kaghad M, Dumont X, Guillemat JC, Le Fur G, Capt D, Ferrero P. (1991) Expression and pharmacological characterization of a human peripheral type benzodiazepine receptor. Eur J Biochem.; 195: 305-11.
  • Stobienia O, Kmita H, Michejda J. (1999) The effect of synthetic polyanion on diffusion of adenine nucleotides through outer membrane of porin-free yeast mitochondria. Biol Bull Poznan.; 36: 17-25.
  • Stocco DM, Clark BJ. (1997) Role of the steroidogenic acute regulatory protein in stereoidogenesis. Steroids.; 62: 29-36.
  • Szewczyk A, Wojtczak L. (2002) Mitochondria as a pharmacological target. Pharmacol Rev.; 54: 101-27.
  • Tatton WG, Olanow CW. (1999) ) Apoptosis in nerodegenerative disease: the role of mitochondria. Biochim Biophys Acta.; 1410: 195-213.
  • Wainright PO, Hinkle G, Sogin ML, Stickel SK. (1993) Monophyletic origins of the metazoa: an evolutionary link with fungi. Science.; 260: 340-2.
  • Weiland GA, Molinoff PB. (1981) Quantitative anlysis of drug-receptor interactions: Determination of kinetic and equilibrium properties. Life Sci.; 29: 313-30.
  • Weizman R, Leschinger S, Schlegel W, Gavish M. (1997) PBR ligands and serum steroid hormones. Brain Res.; 772: 203-8.
  • Zoratti M, Szabo I. (1995) The mitochondrial permeability transition. Biochim Biophys Acta.; 1241: 139-76.
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bwmeta1.element.bwnjournal-article-abpv51i4p953kz
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