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2004 | 51 | 4 | 897-905
Article title

Non-random base composition in codons of mitochondrial cytochrome b gene in vertebrates.

Content
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EN
Abstracts
EN
Cytochrome b is the central catalytic subunit of the quinol:cytochrome c oxidoreductase of complex III of the mitochondrial oxidative phosphorylation system and is essential to the viability of most eukaryotic cells. Partial cytochrome b gene sequences of 14 species representing mammals, birds, reptiles and amphibians are presented here including some species typical for Poland. For the analysed species a comparative analysis of the natural variation in the gene was performed. This information has been used to discuss some aspects of gene sequence - protein function relationships. Review of relevant literature indicates that similar comparisons have been made only for basic mammalian species. Moreover, there is little information about the Polish-specific species. We observed that there is a strong non-random distribution of nucleotides in the cytochrome b sequence in all tested species with the highest differences at the third codon position. This is also the codon position of the strongest compositional bias. Some tested species, representing distant systematic groups, showed unique base composition differing from the others. The quail, frog, python and elk prefer C over A in the light DNA strand. Species belonging to the artiodactyls stand out from the remaining ones and contain fewer pyrimidines. The observed overall rate of amino acid identity is about 61%. The region covering Qo center as well as histidines 82 and 96 (heme ligands) are totally conserved in all tested species. Additionally, the applied method and the sequences can also be used for diagnostic species identification by veterinary and conservation agencies.
Publisher

Year
Volume
51
Issue
4
Pages
897-905
Physical description
Dates
published
2004
received
2004-04-30
revised
2004-09-01
accepted
2004-10-07
Contributors
author
  • Department of Animal Immunogenetics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland
  • The Ludwik Rydygier University School of Medical Sciences, Forensic Medicine Institute, Bydgoszcz, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i4p897kz
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