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2004 | 51 | 4 | 1081-1086
Article title

Enzymatic redox reactions of the explosive 4,6-dinitrobenzofuroxan (DNBF): implications for its toxic action.

Content
Title variants
Languages of publication
EN
Abstracts
EN
With an aim to understand the toxicity mechanisms of the explosive 4,6-dinitro- benzofuroxan (DNBF), we studied its single-electron reduction by NADPH:cytochrome P450 reductase and ferredoxin:NADP+ reductase, and two- electron reduction by DT-diaphorase and Enterobacter cloacae nitroreductase. The enzymatic reactivities of DNBF and another explosive 2,4,6-trinitrotoluene (TNT) were similar, except for the much lower reactivity of DNBF towards nitroreductase. DNBF was less cytotoxic in FLK cells than TNT. However, their action shared the same mechanisms, oxidative stress and activation by DT-diaphorase. The lower cytotoxicity of DNBF may be explained by the negative electrostatic charge of its adduct with water which may impede cellular membrane penetration, and by the formation of its less reactive adducts with intracellular reduced glutathione.
Publisher

Year
Volume
51
Issue
4
Pages
1081-1086
Physical description
Dates
published
2004
received
2004-04-30
revised
2004-07-02
accepted
2004-10-23
Contributors
  • Institute of Immunology, Molėtų Pl. 29, Vilnius 2021, Lithuania
  • Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania
  • Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania
  • Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania
  • Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania
  • Institute of Biochemistry, Mokslininkų 12, Vilnius 2600, Lithuania
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i4p1081kz
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