Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.

• Authors: Ayfer Yalcin
• Languages of publication: EN

Abstracts

EN

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

Keywords

EN

kainic acid; real-time polymerase chain reaction; oxidative stress; thioredoxin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 4
• Pages: 1059-1065

Dates

published

2004

received

2003-10-30

revised

2004-01-15

accepted

2004-09-07

Contributors

author

Ayfer Yalcin

• Department of Biochemistry, Faculty of Pharmacy, Ege University, Izmir, Turkey

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