cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI.

• Authors: Jiayi Zhang , Jianliang Dai , Enpeng Zhao , Yun Lin , Li Zeng , Jinzhong Chen , Huari Zheng , Yu Wang , Xin Li , Kang Ying , Yi Xie , YuMin Mao
• Languages of publication: EN

Abstracts

EN

Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.

Keywords

EN

ovary; ePAD; peptidylarginine deiminase; hPADVI

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 4
• Pages: 1051-1058

Dates

published

2004

received

2004-01-13

revised

2004-10-26

accepted

2004-11-26

Contributors

author

Jiayi Zhang

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Jianliang Dai

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Enpeng Zhao

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Yun Lin

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Li Zeng

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Jinzhong Chen

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Huari Zheng

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Yu Wang

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Xin Li

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Kang Ying

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

Yi Xie

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

author

YuMin Mao

• State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, P. R. China

References

• Guerrin M, Ishigami A, Mechin MC. (2003) Biochem J.; 370: 167-74.
• Imparl JM, Senshu T, Graves DJ. (1995) Arch Biochem Biophys.; 318: 370-7.
• Inagaki M, Takahara H, Nishi Y, Sugawara K, Sato C. (1989) J Biol Chem.; 264: 18119-27.
• Ishigami A, Kuramoto M, Yamada M, Watanabe K, Senshu T. (1998) FEBS Lett.; 433: 113-8.
• Ishigami A, Asaga H, Ohsawa T, Akiyama K, Maruyama N. (2001) Biomed Res.; 22: 63-5.
• Ishigami A, Ohsawa T, Asaga H. (2002) Arch Biochem Biophys.; 407: 25-1.
• Kanno T, Kawada A, Yamanouchi J, et al. (2000) J Invest Dermatol.; 115: 813-23.
• Kozak M. (1987) Nucleic Acids Res.; 15: 8125-48.
• Lamensa JW, Moscarello MA. (1993) J Neurochem.; 61: 987-96.
• Nakashima K, Hagiwara T, Ishigami A. (1999) J Biol Chem.; 274: 27786-2.
• Rothnagel JA, Rogers GE. (1984) Methods Enzymol.; 107: 624-1.
• Tarcsa E, Marekov LN, Mei G, Melino G, Lee SC, Steinert PM. (1996) J Biol Chem.; 271: 30709-6.
• Tarcsa E, Marekov LN, Andreoli J, Idler WW, Candi E, Chung S-I, Steinert PM. (1997) J Biol Chem.; 272: 27893-1.
• Wright PW, Bolling LC, Calvert ME. (2003) Dev Biol.; 256: 73-8.